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A light‐activated acyl carrier protein ‘trap’ for intermediate capture in type II iterative polyketide biocatalysis
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Tosin, Manuela, Kilgour, Samantha Louise, Kilgour, David P. A., Prasongpholchai, Panward and O'Connor, Peter B. (2019) A light‐activated acyl carrier protein ‘trap’ for intermediate capture in type II iterative polyketide biocatalysis. Chemistry – A European Journal, 25 (75). pp. 16515-16518. doi:10.1002/chem.201903662 ISSN 1521-3765.
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WRAP-light-activated-acyl-protein-trap-intermediate-polyketide-Tosin-2019.pdf - Accepted Version - Requires a PDF viewer. Download (1376Kb) | Preview |
Official URL: https://doi.org/10.1002/chem.201903662
Abstract
A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilized for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the type II actinorhodin ‘minimal’ polyketide synthase (PKS), a range of putative ACP‐bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT‐ICR‐MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.
| Item Type: | Journal Article | ||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
| SWORD Depositor: | Library Publications Router | ||||||||
| Journal or Publication Title: | Chemistry – A European Journal | ||||||||
| Publisher: | Wiley | ||||||||
| ISSN: | 1521-3765 | ||||||||
| Official Date: | 20 December 2019 | ||||||||
| Dates: |
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| Volume: | 25 | ||||||||
| Number: | 75 | ||||||||
| Page Range: | pp. 16515-16518 | ||||||||
| DOI: | 10.1002/chem.201903662 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Reuse Statement (publisher, data, author rights): | "This is the peer reviewed version of the following article: Tosin, M. , Kilgour, S. ., Kilgour, D. , Prasongpholchai, P. and O'Connor, P. (2019), A light‐activated acyl carrier protein ‘trap’ for intermediate capture in type II iterative polyketide biocatalysis. Chem. Eur. J., which has been published in final form at https://doi.org/10.1002/chem.201903662 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 7 November 2019 | ||||||||
| Date of first compliant Open Access: | 9 October 2020 |
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