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A light‐activated acyl carrier protein ‘trap’ for intermediate capture in type II iterative polyketide biocatalysis

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Tosin, Manuela, Kilgour, Samantha Louise, Kilgour, David P. A., Prasongpholchai, Panward and O'Connor, Peter B. (2019) A light‐activated acyl carrier protein ‘trap’ for intermediate capture in type II iterative polyketide biocatalysis. Chemistry – A European Journal, 25 (75). pp. 16515-16518. doi:10.1002/chem.201903662

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Official URL: https://doi.org/10.1002/chem.201903662

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Abstract

A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilized for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the type II actinorhodin ‘minimal’ polyketide synthase (PKS), a range of putative ACP‐bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT‐ICR‐MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.

Item Type: Journal Article
Divisions: Faculty of Science > Chemistry
SWORD Depositor: Library Publications Router
Journal or Publication Title: Chemistry – A European Journal
Publisher: Wiley
ISSN: 1521-3765
Official Date: 20 December 2019
Dates:
DateEvent
20 December 2019Published
9 October 2019Available
4 October 2019Accepted
Volume: 25
Number: 75
Page Range: pp. 16515-16518
DOI: 10.1002/chem.201903662
Status: Peer Reviewed
Publication Status: Published
Publisher Statement: "This is the peer reviewed version of the following article: Tosin, M. , Kilgour, S. ., Kilgour, D. , Prasongpholchai, P. and O'Connor, P. (2019), A light‐activated acyl carrier protein ‘trap’ for intermediate capture in type II iterative polyketide biocatalysis. Chem. Eur. J., which has been published in final form at https://doi.org/10.1002/chem.201903662 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
Access rights to Published version: Restricted or Subscription Access

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