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The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis
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Capalbo, Luisa, Bassi, Zuni I., Geymonat, Marco, Todesca, Sofia, Copoiu, Liviu, Enright, Anton J., Callaini, Giuliano, Riparbelli, Maria Giovanna, Yu, Lu, Choudhary, Jyoti S., Ferrero, Enrico, Wheatley, Sally, Douglas, Max E., Mishima, Masanori and D'Avino, Pier Paolo (2019) The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nature Communications, 10 (1). 4513. doi:10.1038/s41467-019-12507-9 ISSN 2041-1723.
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Official URL: https://doi.org/10.1038/s41467-019-12507-9
Abstract
The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cilium and lumen formation. Here, we report the characterization of the intricate midbody protein-protein interaction network (interactome), which identifies many previously unknown interactions and provides an extremely valuable resource for dissecting the multiple roles of the midbody. Initial analysis of this interactome revealed that PP1β-MYPT1 phosphatase regulates microtubule dynamics in late cytokinesis and de-phosphorylates the kinesin component MKLP1/KIF23 of the centralspindlin complex. This de-phosphorylation antagonizes Aurora B kinase to modify the functions and interactions of centralspindlin in late cytokinesis. Our findings expand the repertoire of PP1 functions during mitosis and indicate that spatiotemporal changes in the distribution of kinases and counteracting phosphatases finely tune the activity of cytokinesis proteins.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics | |||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Cytokinesis, Cytology -- Research, Cell division | |||||||||
| Journal or Publication Title: | Nature Communications | |||||||||
| Publisher: | Nature Publishing Group | |||||||||
| ISSN: | 2041-1723 | |||||||||
| Official Date: | 4 October 2019 | |||||||||
| Dates: |
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| Volume: | 10 | |||||||||
| Number: | 1 | |||||||||
| Article Number: | 4513 | |||||||||
| DOI: | 10.1038/s41467-019-12507-9 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 22 January 2020 | |||||||||
| Date of first compliant Open Access: | 22 January 2020 | |||||||||
| RIOXX Funder/Project Grant: |
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