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The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis

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Capalbo, Luisa, Bassi, Zuni I., Geymonat, Marco, Todesca, Sofia, Copoiu, Liviu, Enright, Anton J., Callaini, Giuliano, Riparbelli, Maria Giovanna, Yu, Lu, Choudhary, Jyoti S., Ferrero, Enrico, Wheatley, Sally, Douglas, Max E., Mishima, Masanori and D'Avino, Pier Paolo (2019) The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nature Communications, 10 (1). 4513. doi:10.1038/s41467-019-12507-9 ISSN 2041-1723.

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Official URL: https://doi.org/10.1038/s41467-019-12507-9

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Abstract

The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cilium and lumen formation. Here, we report the characterization of the intricate midbody protein-protein interaction network (interactome), which identifies many previously unknown interactions and provides an extremely valuable resource for dissecting the multiple roles of the midbody. Initial analysis of this interactome revealed that PP1β-MYPT1 phosphatase regulates microtubule dynamics in late cytokinesis and de-phosphorylates the kinesin component MKLP1/KIF23 of the centralspindlin complex. This de-phosphorylation antagonizes Aurora B kinase to modify the functions and interactions of centralspindlin in late cytokinesis. Our findings expand the repertoire of PP1 functions during mitosis and indicate that spatiotemporal changes in the distribution of kinases and counteracting phosphatases finely tune the activity of cytokinesis proteins.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Cytokinesis, Cytology -- Research, Cell division
Journal or Publication Title: Nature Communications
Publisher: Nature Publishing Group
ISSN: 2041-1723
Official Date: 4 October 2019
Dates:
DateEvent
4 October 2019Published
13 September 2019Accepted
Volume: 10
Number: 1
Article Number: 4513
DOI: 10.1038/s41467-019-12507-9
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 22 January 2020
Date of first compliant Open Access: 22 January 2020
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/R001227/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
C309/A25144Cancer Research UKhttp://dx.doi.org/10.13039/501100000289

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