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Tumor protein D54 defines a new class of intracellular transport vesicles
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Larocque, Gabrielle, La-Borde, Penelope J., Clarke, Nicholas I., Carter, Nicholas J. and Royle, Stephen J. (2019) Tumor protein D54 defines a new class of intracellular transport vesicles. Journal of Cell Biology, 219 (1). e201812044. doi:10.1083/jcb.201812044 ISSN 0021-9525.
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Official URL: http://dx.doi.org/10.1083/jcb.201812044
Abstract
Transport of proteins and lipids from one membrane compartment to another is via intracellular vesicles. We investigated the function of tumor protein D54 (TPD54/TPD52L2) and found that TPD54 was involved in multiple membrane trafficking pathways: anterograde traffic, recycling, and Golgi integrity. To understand how TPD54 controls these diverse functions, we used an inducible method to reroute TPD54 to mitochondria. Surprisingly, this manipulation resulted in the capture of many small vesicles (30 nm diameter) at the mitochondrial surface. Super-resolution imaging confirmed the presence of similarly sized TPD54-positive structures under normal conditions. It appears that TPD54 defines a new class of transport vesicle, which we term intracellular nanovesicles (INVs). INVs meet three criteria for functionality. They contain specific cargo, they have certain R-SNAREs for fusion, and they are endowed with a variety of Rab GTPases (16 out of 43 tested). The molecular heterogeneity of INVs and the diverse functions of TPD54 suggest that INVs have various membrane origins and a number of destinations. We propose that INVs are a generic class of transport vesicle that transfer cargo between these varied locations.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) | |||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Tumor proteins, Tumor antigens, Cancer -- Epidemiology, Proteins -- Biological transport | |||||||||||||||
Journal or Publication Title: | Journal of Cell Biology | |||||||||||||||
Publisher: | Rockefeller University Press | |||||||||||||||
ISSN: | 0021-9525 | |||||||||||||||
Official Date: | 31 October 2019 | |||||||||||||||
Dates: |
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Volume: | 219 | |||||||||||||||
Number: | 1 | |||||||||||||||
Article Number: | e201812044 | |||||||||||||||
DOI: | 10.1083/jcb.201812044 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Re-use Statement: | ** Article version: VoR ** From Crossref via Jisc Publications Router ** History: epub 31-10-2019; issued 31-10-2019. ** Licence for VoR version of this article starting on 31-10-2019: https://creativecommons.org/licenses/by/4.0/ | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 17 December 2019 | |||||||||||||||
Date of first compliant Open Access: | 17 December 2019 | |||||||||||||||
RIOXX Funder/Project Grant: |
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