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The S phase checkpoint promotes the Smc5/6 complex dependent SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε.
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Winczura, Alicja, Appanah, Rowin, Tatham, Michael H., Hay, Ronald T. Hay and De Piccoli, Gaicomo (2019) The S phase checkpoint promotes the Smc5/6 complex dependent SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε. PLoS Genetics, 15 (11). e1008427. doi:10.1371/journal.pgen.1008427
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Official URL: https://doi.org/10.1371/journal.pgen.1008427
Abstract
Replication fork stalling and accumulation of single-stranded DNA trigger the S phase checkpoint, a signalling cascade that, in budding yeast, leads to the activation of the Rad53 kinase. Rad53 is essential in maintaining cell viability, but its targets of regulation are still partially unknown. Here we show that Rad53 drives the hyper-SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε, principally following replication forks stalling induced by nucleotide depletion. Pol2 is the main target of SUMOylation within the replisome and its modification requires the SUMO-ligase Mms21, a subunit of the Smc5/6 complex. Moreover, the Smc5/6 complex co-purifies with Pol ε, independently of other replisome components. Finally, we map Pol2 SUMOylation to a single site within the N-terminal catalytic domain and identify a SUMO-interacting motif at the C-terminus of Pol2. These data suggest that the S phase checkpoint regulate Pol ε during replication stress through Pol2 SUMOylation and SUMO-binding ability
| Item Type: | Journal Article | ||||||
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
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| Journal or Publication Title: | PLoS Genetics | ||||||
| Publisher: | Public Library of Science | ||||||
| ISSN: | 1553-7390 | ||||||
| Official Date: | 25 November 2019 | ||||||
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| Date of first compliant deposit: | 11 November 2019 | ||||||
| Volume: | 15 | ||||||
| Number: | 11 | ||||||
| Article Number: | e1008427 | ||||||
| DOI: | 10.1371/journal.pgen.1008427 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access | ||||||
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