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Biochemical characterization of Serpula lacrymans iron-reductase enzymes in lignocellulose breakdown
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Nurika, Irnia, Eastwood, Daniel C., Bugg, Timothy D. H. and Barker, Guy C. (2020) Biochemical characterization of Serpula lacrymans iron-reductase enzymes in lignocellulose breakdown. Journal of Industrial Microbiology & Biotechnology, 47 (1). pp. 145-154. doi:10.1007/s10295-019-02238-7
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Official URL: http://dx.doi.org/10.1007/s10295-019-02238-7
Abstract
Putative iron-reductase (IR) genes from Serpula lacrymans with similarity to the conserved iron-binding domains of cellobiose dehydrogenase (CDH) enzymes have been identified. These genes were cloned and expressed to functionally characterize their activity and role in the decomposition of lignocellulose. The results show that IR1 and IR2 recombinant enzymes have the ability to depolymerize both lignin and cellulose, are capable of the reduction of ferric iron to the ferrous form, and are capable of the degradation of nitrated lignin. Expression of these genes during wheat straw solid-state fermentation was shown to correlate with the release of compounds associated with lignin decomposition. The results suggest that both IR enzymes mediate a non-enzymatic depolymerisation of lignocellulose and highlight the potential of chelator-mediated Fenton systems in the industrial pre-treatment of biomass.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QK Botany | ||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Serpula lacrymans, Lignocellulose, Enzymes, Recombinant DNA -- Research, Biomass energy | ||||||||||||
| Journal or Publication Title: | Journal of Industrial Microbiology & Biotechnology | ||||||||||||
| Publisher: | Springer | ||||||||||||
| ISSN: | 1367-5435 | ||||||||||||
| Official Date: | January 2020 | ||||||||||||
| Dates: |
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| Volume: | 47 | ||||||||||||
| Number: | 1 | ||||||||||||
| Page Range: | pp. 145-154 | ||||||||||||
| DOI: | 10.1007/s10295-019-02238-7 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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