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In situ structure of an intact lipopolysaccharide-bound bacterial surface layer
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von Kügelgen, Andriko, Tang, Haiping, Hardy, Gail G., Kureisaite-Ciziene, Danguole, Brun, Yves V., Stansfeld, Phillip J., Robinson, Carol V. and Bharat, Tanmay A. M. (2020) In situ structure of an intact lipopolysaccharide-bound bacterial surface layer. Cell, 180 (2). P348-358.E15. doi:10.1016/j.cell.2019.12.006 ISSN 0092-8674.
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WRAP-structural-basis-lipopolysaccharide-mediated-surface-layer-anchoring-Caulobacter-crescentus-cells-Stansfeld-2019.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (37Mb) |
Official URL: https://doi.org/10.1016/j.cell.2019.12.006
Abstract
Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. Using native mass spectrometry and molecular dynamics simulations, we deduce the length of the O-antigen on cells and show how lipopolysaccharide binding and S-layer assembly is regulated by calcium. Finally, we present a near-atomic resolution in situ structure of the complete S-layer using cellular electron cryotomography, showing S-layer arrangement at the tip of the O-antigen. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications.
Item Type: | Journal Article | ||||||||||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||
Journal or Publication Title: | Cell | ||||||||||||||||||
Publisher: | Cell Press | ||||||||||||||||||
ISSN: | 0092-8674 | ||||||||||||||||||
Official Date: | 23 January 2020 | ||||||||||||||||||
Dates: |
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Volume: | 180 | ||||||||||||||||||
Number: | 2 | ||||||||||||||||||
Page Range: | P348-358.E15 | ||||||||||||||||||
DOI: | 10.1016/j.cell.2019.12.006 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||
Date of first compliant deposit: | 12 December 2019 | ||||||||||||||||||
Date of first compliant Open Access: | 26 March 2020 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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