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Nano-encapsulated escherichia coli divisome anchor ZipA, and in complex with FtsZ

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Lee, Sarah C., Collins, Richard, Lin, Yu-pin, Jamshad, Mohammed, Broughton, Claire E., Harris, Sarah A., Hanson, Benjamin S, Tognoloni, Cecilia, Parslow, Rosemary A., Terry, Ann E., Rodger, Alison, Smith, Corinne J., Edler, Karen J., Ford, Robert, Roper, David I. and Dafforn, Timothy R. (2019) Nano-encapsulated escherichia coli divisome anchor ZipA, and in complex with FtsZ. Scientific Reports, 9 (1). 18712. doi:10.1038/s41598-019-54999-x

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Official URL: http://dx.doi.org/10.1038/s41598-019-54999-x

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Abstract

The E. coli membrane protein ZipA, binds to the tubulin homologue FtsZ, in the early stage of cell division. We isolated ZipA in a Styrene Maleic Acid lipid particle (SMALP) preserving its position and integrity with native E. coli membrane lipids. Direct binding of ZipA to FtsZ is demonstrated, including FtsZ fibre bundles decorated with ZipA. Using Cryo-Electron Microscopy, small-angle X-ray and neutron scattering, we determine the encapsulated-ZipA structure in isolation, and in complex with FtsZ to a resolution of 1.6 nm. Three regions can be identified from the structure which correspond to, SMALP encapsulated membrane and ZipA transmembrane helix, a separate short compact tether, and ZipA globular head which binds FtsZ. The complex extends 12 nm from the membrane in a compact structure, supported by mesoscale modelling techniques, measuring the movement and stiffness of the regions within ZipA provides molecular scale analysis and visualisation of the early divisome.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QP Physiology
Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Escherichia coli, Membrane proteins -- Research, Cell division -- Research, Lipids -- Research
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Official Date: 10 December 2019
Dates:
DateEvent
10 December 2019Published
10 November 2019Accepted
Volume: 9
Number: 1
Article Number: 18712
DOI: 10.1038/s41598-019-54999-x
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/J017310/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

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