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Basal rot of narcissus : understanding pathogenicity in fusarium oxysporum f. sp. narcissi

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Taylor, Andrew, Armitage, Andrew D., Handy, Claire, Jackson, Alison C., Hulin, Michelle T., Harrison, Richard J. and Clarkson, John P. (2019) Basal rot of narcissus : understanding pathogenicity in fusarium oxysporum f. sp. narcissi. Frontiers in Microbiology, 10 . 2905. doi:10.3389/fmicb.2019.02905

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Official URL: https://doi.org/10.3389/fmicb.2019.02905

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Abstract

Fusarium oxysporum is a globally distributed soilborne fungal pathogen causing root rots, bulb rots, crown rots and vascular wilts on a range of horticultural plants. Pathogenic F. oxysporum isolates are highly host specific and are classified as formae speciales. Narcissus is an important ornamental crop and both the quality and yield of flowers and bulbs can be severely affected by a basal rot caused by F. oxysporum f. sp. narcissi (FON); 154 Fusarium isolates were obtained from different locations and Narcissus cultivars in the United Kingdom, representing a valuable resource. A subset of 30 F. oxysporum isolates were all found to be pathogenic and were therefore identified as FON. Molecular characterisation of isolates through sequencing of three housekeeping genes, suggested a monophyletic origin with little divergence. PCR detection of 14 Secreted in Xylem (SIX) genes, previously shown to be associated with pathogenicity in other F. oxysporum f. spp., revealed different complements of SIX7, SIX9, SIX10, SIX12 and SIX13 within FON isolates which may suggest a race structure. SIX gene sequences were unique to FON and SIX10 was present in all isolates, allowing for molecular identification of FON for the first time. The genome of a highly pathogenic isolate was sequenced and lineage specific (LS) regions identified which harboured putative effectors including the SIX genes. Real-time RT-PCR, showed that SIX genes and selected putative effectors were expressed in planta with many significantly upregulated during infection. This is the first study to characterise molecular variation in FON and provide an analysis of the FON genome. Identification of expressed genes potentially associated with virulence provides the basis for future functional studies and new targets for molecular diagnostics.

Item Type: Journal Article
Subjects: Q Science > QK Botany
Q Science > QR Microbiology
S Agriculture > SB Plant culture
Divisions: Faculty of Science > Life Sciences (2010- )
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Fusarium oxysporum, Narcissus bulbs , Pathogenic fungi, Bulbs (Plants) -- Diseases and pests, Xylem
Journal or Publication Title: Frontiers in Microbiology
Publisher: Frontiers Media S.A.
ISSN: 1664-302X
Official Date: 19 December 2019
Dates:
DateEvent
19 December 2019Published
2 December 2019Accepted
Volume: 10
Article Number: 2905
DOI: 10.3389/fmicb.2019.02905
Status: Peer Reviewed
Publication Status: Published
Publisher Statement: ** From Frontiers via Jisc Publications Router ** History: collection 2019; received 21-08-2019; accepted 02-12-2019; epub 19-12-2019. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB K020870 and BB K020730[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BOF 74 and FV POBOF 452Agriculture and Horticulture Development Boardhttp://dx.doi.org/10.13039/100008123
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