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Basal rot of narcissus : understanding pathogenicity in fusarium oxysporum f. sp. narcissi
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Taylor, Andrew, Armitage, Andrew D., Handy, Claire, Jackson, Alison C., Hulin, Michelle T., Harrison, Richard J. and Clarkson, John P. (2019) Basal rot of narcissus : understanding pathogenicity in fusarium oxysporum f. sp. narcissi. Frontiers in Microbiology, 10 . 2905. doi:10.3389/fmicb.2019.02905
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Official URL: https://doi.org/10.3389/fmicb.2019.02905
Abstract
Fusarium oxysporum is a globally distributed soilborne fungal pathogen causing root rots, bulb rots, crown rots and vascular wilts on a range of horticultural plants. Pathogenic F. oxysporum isolates are highly host specific and are classified as formae speciales. Narcissus is an important ornamental crop and both the quality and yield of flowers and bulbs can be severely affected by a basal rot caused by F. oxysporum f. sp. narcissi (FON); 154 Fusarium isolates were obtained from different locations and Narcissus cultivars in the United Kingdom, representing a valuable resource. A subset of 30 F. oxysporum isolates were all found to be pathogenic and were therefore identified as FON. Molecular characterisation of isolates through sequencing of three housekeeping genes, suggested a monophyletic origin with little divergence. PCR detection of 14 Secreted in Xylem (SIX) genes, previously shown to be associated with pathogenicity in other F. oxysporum f. spp., revealed different complements of SIX7, SIX9, SIX10, SIX12 and SIX13 within FON isolates which may suggest a race structure. SIX gene sequences were unique to FON and SIX10 was present in all isolates, allowing for molecular identification of FON for the first time. The genome of a highly pathogenic isolate was sequenced and lineage specific (LS) regions identified which harboured putative effectors including the SIX genes. Real-time RT-PCR, showed that SIX genes and selected putative effectors were expressed in planta with many significantly upregulated during infection. This is the first study to characterise molecular variation in FON and provide an analysis of the FON genome. Identification of expressed genes potentially associated with virulence provides the basis for future functional studies and new targets for molecular diagnostics.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QK Botany Q Science > QR Microbiology S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Fusarium oxysporum, Narcissus bulbs , Pathogenic fungi, Bulbs (Plants) -- Diseases and pests, Xylem | |||||||||
| Journal or Publication Title: | Frontiers in Microbiology | |||||||||
| Publisher: | Frontiers Media S.A. | |||||||||
| ISSN: | 1664-302X | |||||||||
| Official Date: | 19 December 2019 | |||||||||
| Dates: |
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| Date of first compliant deposit: | 3 January 2020 | |||||||||
| Volume: | 10 | |||||||||
| Article Number: | 2905 | |||||||||
| DOI: | 10.3389/fmicb.2019.02905 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Publisher Statement: | ** From Frontiers via Jisc Publications Router ** History: collection 2019; received 21-08-2019; accepted 02-12-2019; epub 19-12-2019. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| RIOXX Funder/Project Grant: |
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