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Transmembrane peptide 4 and 5 of APJ are essential for its heterodimerization with OX1R

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Wan, Lei, Xu, Fangfang, Liu, Chang, Ji, Bingyuan, Zhang, Rumin, Wang, Peixiang, Wu, Fei, Pan, Yanyou, Yang, Chunqing, Wang, Chunmei and Chen, Jing (2020) Transmembrane peptide 4 and 5 of APJ are essential for its heterodimerization with OX1R. Biochemical and Biophysical Research Communications, 521 (2). pp. 408-413. doi:10.1016/j.bbrc.2019.10.146 ISSN 0006-291X.

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Official URL: https://doi.org/10.1016/j.bbrc.2019.10.146

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Abstract

Increasing evidence indicates some G protein-coupled receptors function as a heterodimer, which provide a novel target for therapeutics investigation. However, study on the receptor-receptor interaction interface, a potent target on interfering dimer formation, are still limited. Here, using bioluminescence resonance energy transfer (BRET) combined with co-immunoprecipitation (Co-IP), we found a new constitutive GPCR heterodimer, apelin receptor (APJ)-orexin receptor type 1 (OX1R). Both APJ and OX1R co-internalized when constantly subjected to cognate agonist (apelin-13 or orexin-A) specific to either protomer. Combined with BRET and immunostaining, the in vitro synthesized transmembrane peptides (TMs) interfering experiments suggests that TM4 and 5 of APJ act as the interaction interface of the APJ-OX1R heterodimer, and co-internalization could be disrupted by these peptides as well. Our study not only provide new evidence on GPCR heterodimerization, but address a novel heterodimerization interface, which can be severed as a potential pharmacological target.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Peptides, G proteins -- Receptors -- Research, Orexins, Bioluminescence, Membrane proteins -- Research
Journal or Publication Title: Biochemical and Biophysical Research Communications
Publisher: Elsevier
ISSN: 0006-291X
Official Date: 8 January 2020
Dates:
DateEvent
8 January 2020Published
25 December 2019Available
21 October 2019Accepted
Volume: 521
Number: 2
Page Range: pp. 408-413
DOI: 10.1016/j.bbrc.2019.10.146
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 10 January 2020
Date of first compliant Open Access: 25 December 2020
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
ZR201702210478Natural Science Foundation of Shandong Provincehttp://dx.doi.org/10.13039/501100007129
ZR2017BH056Natural Science Foundation of Shandong Provincehttp://dx.doi.org/10.13039/501100007129

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