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Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations
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Cassidy, Keith, Himes, Benjamin A., Sun, Dapeng, Ma, Jun, Zhao, Gongpu, Parkinson, John S., Stansfeld, Phillip J., Luthey-Schulten, Z. and Zhang, Peijun (2020) Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations. Communications Biology, 3 (24). doi:10.1038/s42003-019-0748-0
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Official URL: http://dx.doi.org/10.1038/s42003-019-0748-0
Abstract
To enable the processing of chemical gradients, chemotactic bacteria possess large arrays of transmembrane chemoreceptors, the histidine kinase CheA, and the adaptor protein CheW, organized as coupled core-signaling units (CSU). Despite decades of study, important questions surrounding the molecular mechanisms of sensory signal transduction remain unresolved, owing especially to the lack of a high-resolution CSU structure. Here, we use cryo-electron tomography and sub-tomogram averaging to determine a structure of the Escherichia coli CSU at sub-nanometer resolution. Based on our experimental data, we use molecular simulations to construct an atomistic model of the CSU, enabling a detailed characterization of CheA conformational dynamics in its native structural context. We identify multiple, distinct conformations of the critical P4 domain as well as asymmetries in the localization of the P3 bundle, offering several novel insights into the CheA signaling mechanism.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Chemoreceptors, Signal transducing adaptor proteins, Molecular biology, Cellular signal transduction, Escherichia coli | ||||||
| Journal or Publication Title: | Communications Biology | ||||||
| Publisher: | Nature Publishing Group | ||||||
| ISSN: | 2399-3642 | ||||||
| Official Date: | 10 January 2020 | ||||||
| Dates: |
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| Date of first compliant deposit: | 3 January 2020 | ||||||
| Volume: | 3 | ||||||
| Number: | 24 | ||||||
| DOI: | 10.1038/s42003-019-0748-0 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access | ||||||
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