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Light microscopy in trypanosomes : use of fluorescent proteins and tags
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Dean, Samuel and Sunter, Jack (2020) Light microscopy in trypanosomes : use of fluorescent proteins and tags. In: Michels, P. and Ginger, M. and Zilberstein, D., (eds.) Trypanosomatids. Methods in Molecular Biology, 2116 . New York, NY.: Humana Press, pp. 367-383. ISBN 9781071602935
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Official URL: https://doi.org/10.1007/978-1-0716-0294-2_23
Abstract
Fluorescence microscopy enables the localization of proteins to specific structures within a cell which have either been fused to a fluorescence protein or detected by immunofluorescence. Here, we describe the various procedures that can be used to prepare both the procyclic form and bloodstream form of the human pathogen Trypanosoma brucei for fluorescence microscopy. The choice of procedure to be used is determined by various parameters, including protein characteristics and the scientific question being investigated.
| Item Type: | Book Item | ||||||
|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Series Name: | Methods in Molecular Biology | ||||||
| Publisher: | Humana Press | ||||||
| Place of Publication: | New York, NY. | ||||||
| ISBN: | 9781071602935 | ||||||
| Book Title: | Trypanosomatids | ||||||
| Editor: | Michels, P. and Ginger, M. and Zilberstein, D. | ||||||
| Official Date: | 28 March 2020 | ||||||
| Dates: |
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| Volume: | 2116 | ||||||
| Page Range: | pp. 367-383 | ||||||
| DOI: | 10.1007/978-1-0716-0294-2_23 | ||||||
| Status: | Not Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
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