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Stratification relieves constraints from steric hindrance in the generation of compact acto-myosin asters at the membrane cortex

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Das, Amit, Bhat, Abrar, Sknepnek, Rastko, Koester, Darius, Mayor, Satyajit and Rao, Madan (2020) Stratification relieves constraints from steric hindrance in the generation of compact acto-myosin asters at the membrane cortex. Science Advances, 6 (11). eaay6093. doi:10.1126/sciadv.aay6093 ISSN 2375-2548.

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Official URL: https://doi.org/10.1126/sciadv.aay6093

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Abstract

Recent in-vivo studies have revealed that several membrane proteins are driven to form nanoclusters by active contractile flows arising from F-actin and myosin at the cortex. The mechanism of clustering was shown to be arising from the dynamic patterning of transient contractile platforms (asters) generated by actin and myosin. Myosin-II, which assemble as minifilaments consisting of tens of myosin heads, are rather bulky structures and hence a concern could be that steric considerations might obstruct the emergence of nanoclustering. Here, using coarse-grained, agent-based simulations that respect the size of constituents, we find that in the presence of steric hindrance, the patterns exhibited by actomyosin in two dimensions, do not resemble the steady state patterns observed in our in-vitro reconstitution of actomyosin on a supported bilayer. We then perform simulations in a thin rectangular slab, allowing the separation of a layer of actin filaments from those of myosin-II minifilaments. This recapitulates the observed features of in-vitro patterning. Using super resolution microscopy, we find direct evidence for stratification in our in-vitro system. Our study suggests the possibility that molecular stratification may be an important organising feature of the cortical cytoskeleton in-vivo.

Item Type:

Journal Article

Subjects:

Q Science > QC Physics

Divisions:

Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School

Journal or Publication Title:

Science Advances

Publisher:

American Association for the Advancement of Science

ISSN:

2375-2548

Official Date:

11 March 2020

Dates:

Date	Event
11 March 2020	Available
4 December 2019	Accepted

Volume:

Number:

Article Number:

eaay6093

DOI:

10.1126/sciadv.aay6093

Status:

Peer Reviewed

Publication Status:

Published

Access rights to Published version:

Open Access (Creative Commons)

Date of first compliant deposit:

6 February 2020

Date of first compliant Open Access:

13 March 2020

Funder:

SM and MR acknowledge a JC Bose Fellowship from DST (Government of India), and support from the NCBS-Max Planck Lipid Centre, SM acknowledges HFSP (RGP0027/2012), and Margadarshi fellowship (IA/M/15/1/502018) from The Wellcome Trust-DBT India Alliance

RIOXX Funder/Project Grant:

Project/Grant ID	RIOXX Funder Name	Funder ID
BB/N009789/1	[BBSRC] Biotechnology and Biological Sciences Research Council	http://dx.doi.org/10.13039/501100000268
RGP0027/2012	Wellcome Trust	http://dx.doi.org/10.13039/100010269
IA/M/15/1/502018	The Wellcome Trust DBT India Alliance	http://dx.doi.org/10.13039/501100009053