Molecular biology and regulation of methane monooxygenase
UNSPECIFIED (2000) Molecular biology and regulation of methane monooxygenase. ARCHIVES OF MICROBIOLOGY, 173 (5-6). pp. 325-332. ISSN 0302-8933Full text not available from this repository.
Methanotrophs are ubiquitous in the environment and play an important role in mitigating global warming due to methane. They are also potentially interesting for industrial applications such as production of bulk chemicals or bioremediation. The first step in the oxidation of methane is the conversion to methanol by methane monooxygenase, the key enzyme, which exists in two forms: the cytoplasmic, soluble methane monooxygenase (sMMO) and the membrane-bound, particulate methane monooxygenase (pMMO). This paper reviews the biochemistry and molecular biology of both forms of MMO. In the past few years there have been many exciting new findings. sMMO components have been expressed in heterologous and homologous hosts. The pMMO has been purified and biochemically studied in some detail and the genes encoding the pMMO have been sequenced. Copper ions have been shown to play a key role in regulating the expression of both MMO enzyme complexes. We also present a model for copper regulation based on results from Northern analysis, primer-extensions and new sequence data, and raise a number of unanswered questions for future studies.
|Item Type:||Journal Item|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||ARCHIVES OF MICROBIOLOGY|
|Official Date:||May 2000|
|Number of Pages:||8|
|Page Range:||pp. 325-332|
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