Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release

Tools
- Tools
+ Tools

Tagliatti, Erica, Bello, Oscar D., Mendonça, Philipe R. F., Kotzadimitriou, Dimitrios, Nicholson, Elizabeth, Coleman, Jeff, Timofeeva, Yulia, Rothman, James E., Krishnakumar, Shyam S. and Volynski, Kirill E. (2020) Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release. Proceedings of the National Academy of Sciences . 201920403. doi:10.1073/pnas.1920403117 (In Press)

[img]
Preview
PDF
WRAP-Synaptotagmin-1-oligomers-clamp-regulate-different-modes-neurotransmitter-release-Timofeeva-2020.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0.

Download (1884Kb) | Preview
Official URL: http://dx.doi.org/10.1073/pnas.1920403117

Request Changes to record.

Abstract

Release of neurotransmitters relies on submillisecond coupling of synaptic vesicle fusion to the triggering signal: AP-evoked presynaptic Ca2+ influx. The key player that controls exocytosis of the synaptic vesicle is the Ca2+ sensor synaptotagmin 1 (Syt1). While the Ca2+ activation of Syt1 has been extensively characterized, how Syt1 reversibly clamps vesicular fusion remains enigmatic. Here, using a targeted mutation combined with fluorescence imaging and electrophysiology, we show that the structural feature of Syt1 to self-oligomerize provides the molecular basis for clamping of spontaneous and asynchronous release but is not required for triggering of synchronous release. Our findings propose a mechanistic model that explains how Syt1 oligomers regulate different modes of transmitter release in neuronal synapses.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Science > Centre for Complexity Science
Faculty of Science > Computer Science
Library of Congress Subject Headings (LCSH): Neural transmission -- Regulation, Neurotransmitters, Synaptic vesicles, Synaptotagmin-1
Journal or Publication Title: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
ISSN: 0027-8424
Official Date: 3 February 2020
Dates:
DateEvent
3 February 2020Published
20 December 2020Accepted
Date of first compliant deposit: 11 February 2020
Article Number: 201920403
DOI: 10.1073/pnas.1920403117
Status: Peer Reviewed
Publication Status: In Press
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDWellcome Trusthttp://dx.doi.org/10.13039/100010269
UNSPECIFIED[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us