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Combinatorial biomaterials discovery strategy to identify new macromolecular cryoprotectants
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Stubbs, Christopher D., Murray, Kathryn, Ishibe, Toru, Mathers, Robert T. and Gibson, Matthew I. (2020) Combinatorial biomaterials discovery strategy to identify new macromolecular cryoprotectants. ACS Macro Letters, 9 (2). pp. 290-294. doi:10.1021/acsmacrolett.0c00044 ISSN 2161-1653.
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Official URL: https://doi.org/10.1021/acsmacrolett.0c00044
Abstract
Cryoprotective agents (CPAs) are typically solvents or small molecules, but there is a need for innovative CPAs to reduce toxicity and increase cell yield, for the banking and transport of cells. Here we use a photochemical high-throughput discovery platform to identify macromolecular cryoprotectants, as rational design approaches are currently limited by the lack of structure–property relationships. Using liquid handling systems, 120 unique polyampholytes were synthesized using photopolymerization with RAFT agents. Cryopreservation screening identified “hit” polymers and nonlinear trends between composition and function, highlighting the requirement for screening, with polymer aggregation being a key factor. The most active polymers reduced the volume of dimethyl sulfoxide (DMSO) required to cryopreserve a nucleated cell line, demonstrating the potential of this approach to identify materials for cell storage and transport.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QD Chemistry | |||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||
| SWORD Depositor: | Library Publications Router | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cryochemistry, Combinatorial chemistry, Polyampholytes | |||||||||||||||
| Journal or Publication Title: | ACS Macro Letters | |||||||||||||||
| Publisher: | American Chemical Society (ACS) | |||||||||||||||
| ISSN: | 2161-1653 | |||||||||||||||
| Official Date: | 18 February 2020 | |||||||||||||||
| Dates: |
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| Volume: | 9 | |||||||||||||||
| Number: | 2 | |||||||||||||||
| Page Range: | pp. 290-294 | |||||||||||||||
| DOI: | 10.1021/acsmacrolett.0c00044 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Copyright Holders: | Copyright © 2020 American Chemical Society | |||||||||||||||
| Date of first compliant deposit: | 5 May 2020 | |||||||||||||||
| Date of first compliant Open Access: | 5 May 2020 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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