Gibson, Matthew I. and Tomás, Ruben M. F. (2019) Data for Optimization and stability of cell–polymer hybrids obtained by “clicking” synthetic polymers to metabolically labeled cell surface glycans. [Dataset]

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Abstract

Re-engineering of mammalian cell surfaces with polymers enables the introduction of functionality including imaging agents, drug cargoes or antibodies for cell-based therapies, without resorting to genetic techniques. Glycan metabolic labeling has been reported as a tool for engineering cell surface glycans with synthetic polymers through the installation of biorthogonal handles, such as azides. Quantitative assessment of this approach and the robustness of the engineered coatings has yet to be explored. Here, we graft poly(hydroxyethyl acrylamide) onto azido-labeled cell surface glycans using strain-promoted azide–alkyne “click” cycloaddition and, using a combination of flow cytometry and confocal microscopy, evaluate the various parameters controlling the outcome of this “grafting to” process. In all cases, homogeneous cell coatings were formed with >95% of the treated cells being covalently modified, superior to nonspecific “grafting to” approaches. Controllable grafting densities could be achieved through modulation of polymer chain length and/or concentration, with longer polymers having lower densities. Cell surface bound polymers were retained for at least 72 h, persisting through several mitotic divisions during this period. Furthermore, we postulate that glycan/membrane recycling is slowed by the steric bulk of the polymers, demonstrating robustness and stability even during normal biological processes. This cytocompatible, versatile and simple approach shows potential for re-engineering of cell surfaces with new functionality for future use in cell tracking or cell-based therapies.

Item Type:	Dataset
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Chemistry
Type of Data:	Experimental data
Library of Congress Subject Headings (LCSH):	Cell membranes, Glycoconjugates, T cells -- Receptors, Addition polymerization
Publisher:	University of Warwick, Department of Chemistry
Official Date:	29 May 2019
Dates:	Date Event 29 May 2019 Published
Status:	Not Peer Reviewed
Publication Status:	Published
Media of Output (format):	.xlsx
Access rights to Published version:	Open Access (Creative Commons)
Copyright Holders:	University of Warwick
Description:	--> Software required Microsoft excel --> Data organisation Single folder containing background data for flow cytometry, SEC, NMR, and enzymatic assays under appropriate worksheets.
Date of first compliant deposit:	26 February 2020
Date of first compliant Open Access:	26 February 2020
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID CRYOMAT 638661 European Research Council http://dx.doi.org/10.13039/501100000781 PoC Grant (789182) European Research Council http://dx.doi.org/10.13039/501100000781 EP/L015307/1RT [EPSRC] Engineering and Physical Sciences Research Council http://dx.doi.org/10.13039/501100000266 BB/ M01228X/1 [BBSRC] Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268
Related URLs:	Related item in WRAP Other
Contributors:	Contribution Name Contributor ID Depositor Gibson, Matthew I. 32015

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