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Low temperature stimulates spatial molecular reprogramming of the Arabidopsis seed germination programme
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Mitchell, Jack, Mukhtar, Nur K. and Bassel, George (2021) Low temperature stimulates spatial molecular reprogramming of the Arabidopsis seed germination programme. Seed Science Research, 30 (1). pp. 2-12. doi:10.1017/S0960258519000266 ISSN 0960-2585.
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Official URL: http://dx.doi.org/10.1017/S0960258519000266
Abstract
The timing of the germination of seeds is highly responsive to inputs from the environment. Temperature plays a key role in the control of germination, with low temperatures acting to stimulate this developmental transition in many species. In Arabidopsis, extensive gene expression changes have been reported at the whole seed level in response to cold, while much less is known about their spatial distribution across the diverse cell types of the embryo. In this study we examined the spatiotemporal patterns of promoter activity and protein abundance for key gibberellic acid (GA) and abscisic acid (ABA) factors which regulate the decision to germinate both during a time course of germination and in response to cold. Low temperature stimulated the spatial relocalization of these factors to the vasculature. The response of these seeds to dormancy-breaking stratification treatments therefore stimulates the distribution of both positive (GA) and negatively acting (ABA) components to this same cell type. This altered spatial pattern persisted following the transfer of seeds to 22°C, as well as after their rehydration, indicating that this alteration is persistent. These observations suggest that the vasculature plays a role in the low temperature-mediated stimulation of germination in this species, while novel cell types are recruited to promote germination in response to stratification.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Abscisic acid, Gibberellins , Germination , Seeds -- Stratification | ||||||||||||||||||
Journal or Publication Title: | Seed Science Research | ||||||||||||||||||
Publisher: | Cambridge University Press | ||||||||||||||||||
ISSN: | 0960-2585 | ||||||||||||||||||
Official Date: | March 2021 | ||||||||||||||||||
Dates: |
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Volume: | 30 | ||||||||||||||||||
Number: | 1 | ||||||||||||||||||
Page Range: | pp. 2-12 | ||||||||||||||||||
DOI: | 10.1017/S0960258519000266 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Copyright Holders: | © The Author(s) 2020 | ||||||||||||||||||
Date of first compliant deposit: | 5 March 2020 | ||||||||||||||||||
Date of first compliant Open Access: | 10 March 2020 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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