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Polar relaxation by dynein-mediated removal of cortical myosin II
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Chapa-y-Lazo, Bernardo, Hamanaka, Motonari, Wray, Alexander, Balasubramanian, Mohan K. and Mishima, Masanori (2020) Polar relaxation by dynein-mediated removal of cortical myosin II. Journal of Cell Biology . doi:10.1083/jcb.201903080 (In Press)
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Official URL: https://doi.org/10.1083/jcb.201903080
Abstract
Nearly 6 decades ago, Lewis Wolpert proposed the relaxation of the polar cell cortex by the radial arrays of astral microtubules as a mechanism for cleavage furrow induction (White and Borisy, 1983; Wolpert, 1960). While this mechanism has remained controversial (Rappaport, 1996), recent work has provided evidence for polar relaxation by astral microtubules (Chen et al., 2008; Dechant and Glotzer, 2003; Foe and Dassow, 2008; Murthy and Wadsworth, 2008; Werner et al., 2007), although its molecular mechanisms remain elusive. Here, using C. elegans embryos, we show that polar relaxation is achieved through dynein-mediated removal of myosin II from the polar cortexes. Mutants that position centrosomes closer to the polar cortex accelerated furrow induction whereas suppression of dynein activity delayed furrowing. We provide evidence that dynein-mediated removal of myosin II from the polar cortexes triggers cortical flow towards the cell equator, which induces the assembly of the actomyosin contractile ring. These studies for the first time provide a molecular basis for the aster-dependent polar relaxation, which works in parallel with equatorial stimulation to promote robust cytokinesis.
| Item Type: | Journal Article | ||||||
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
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| Journal or Publication Title: | Journal of Cell Biology | ||||||
| Publisher: | Rockefeller University Press | ||||||
| ISSN: | 0021-9525 | ||||||
| Book Title: | Polar relaxation by dynein-mediated removal of cortical myosin II | ||||||
| Official Date: | 4 May 2020 | ||||||
| Dates: |
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| Date of first compliant deposit: | 11 May 2020 | ||||||
| DOI: | 10.1083/jcb.201903080 | ||||||
| Institution: | University of Warwick | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | In Press | ||||||
| Publisher Statement: | © 2020 Chapa-y-Lazo et al. Originally published in Journal of Cell Biology. https://doi.org/10.1083/jcb.201903080. | ||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||
| Copyright Holders: | The Authors | ||||||
| Open Access Version: |
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