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Properties of a neutralizing antibody that recognizes a conformational form of epitope ERDRD in the gp41 C-terminal tail of human immunodeficiency virus type 1
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UNSPECIFIED (2000) Properties of a neutralizing antibody that recognizes a conformational form of epitope ERDRD in the gp41 C-terminal tail of human immunodeficiency virus type 1. JOURNAL OF GENERAL VIROLOGY, 81 (Part 5). pp. 1251-1260. ISSN 0022-1317.
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Abstract
The possibility that epitopes from the C-terminal tail of the gp41 transmembrane protein of human immunodeficiency virus type 1 (HIV-1) are exposed the surface of the virion has long been contentious. Resolution of this has been hampered by the absence of any neutralizing monoclonal antibodies, but we have recently epitope-purified a neutralizing polyclonal IgG specific for one of the putative gp41 tail epitopes, (ERDRD750)-E-746. This was Obtained from mice immunized parenterally with a plant virus chimera expressing residues 731-752 from the gp41 tail. The ERDRD epitope is highly conformational and is conserved in 81% of B clade viruses. Here, it is shown that this polyclonal ERDRD-specific IgG is highly potent, with an affinity of 2.2 x 10(8) M-1, and a neutralization rate constant (-K-neut) of 7.8 x 10(4) M-1 s(-1) that exceeds that of nearly all other known HIV-l-neutralizing antibodies. ERDRD-specific IgG gave 50% neutralization at 0.1-0.2 mu g/ml and 90% neutralization at approximately 3 mu g/ml. It also neutralized virus that was already attached to target cells, and this and other data suggest that it neutralized by inhibiting a virion event that precedes the fusion-entry process. Consistent with this conclusion was the finding that neutralizing amounts of ERDRD-specific IgG did not inhibit the attachment of free virus to target cells. ERDRD-specific IgG was also cross-reactive and neutralized all but one of six B clade T cell line-adapted strains tested.
| Item Type: | Journal Article | ||||
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| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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| Journal or Publication Title: | JOURNAL OF GENERAL VIROLOGY | ||||
| Publisher: | SOC GENERAL MICROBIOLOGY | ||||
| ISSN: | 0022-1317 | ||||
| Official Date: | May 2000 | ||||
| Dates: |
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| Volume: | 81 | ||||
| Number: | Part 5 | ||||
| Number of Pages: | 10 | ||||
| Page Range: | pp. 1251-1260 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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