Berkhout, Daniel J. C., Niemarkt, Hendrik J., Andriessen, Peter, Vijlbrief, Daniel C., Bomers, Marije K., Cossey, Veerle, Hulzebos, Christian V., van Kaam, Anton H., Kramer, Boris W., van Lingen, Richard A., Wicaksono, Alfian, Covington, James A., van Weissenbruch, Mirjam M., Benninga, Marc A., de Boer, Nanne K. H. and de Meij, Tim G. J. (2020) Preclinical detection of non-catheter related late-onset sepsis in preterm infants by fecal volatile compounds analysis : a prospective, multi-center cohort study. The Pediatric Infectious Disease Journal . doi:10.1097/INF.0000000000002589 (In Press)

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Abstract

Background:
Late onset sepsis (LOS) in preterm infants is preceded by fecal volatile organic compound (VOC) alterations, suggesting an etiological role of gut microbiota in LOS rather than being primarily caused by central venous catheters (CVC). To increase our knowledge about the involvement of the gut microbiota in LOS, we analyzed fecal samples from septic infants without a CVC.
Methods:
In this prospective multicenter study, fecal samples were collected daily from all infants born at ≤30 weeks gestation. Fecal VOC profiles up to 3 days prior to sepsis onset from infants with non-catheter–related LOS were compared with profiles from non-septic controls by means of High-Field Asymmetric Waveform Ion Mobility Spectrometry.
Results:
In total, 104 fecal samples were analyzed. Fecal VOC profiles allowed for discrimination between non-catheter–related LOS cases (n = 24) and matched controls (n = 25). Discriminative accuracy increased after focusing on center of origin (area under the curve, sensitivity, specificity; 0.95, 100%, 83%) and after focusing on LOS cases caused by Staphylococcus epidermidis (0.95, 100%, 78%), the most cultured pathogen (n = 11).
Conclusions:
Fecal VOC profiles of preterm LOS infants without a CVC differed from matched controls underlining the increasing notion that aberrations in gut microbiota composition and activity may play a role in LOS etiology.

Item Type:	Journal Article
Subjects:	R Medicine > RB Pathology R Medicine > RC Internal medicine R Medicine > RJ Pediatrics
Divisions:	Faculty of Science > Engineering
Library of Congress Subject Headings (LCSH):	Septicemia, Premature infants, Feces -- Examination
Journal or Publication Title:	The Pediatric Infectious Disease Journal
Publisher:	Lippincott Williams & Wilkins
ISSN:	1532-0987
Official Date:	6 February 2020
Dates:	Date Event 6 February 2020 Available 6 March 2020 Accepted
Date of first compliant deposit:	12 March 2020
DOI:	10.1097/INF.0000000000002589
Status:	Peer Reviewed
Publication Status:	In Press
Publisher Statement:	This is a non-final version of an article published in final form in Berkhout, Daniel J. C., Niemarkt, Hendrik J., Andriessen, Peter, Vijlbrief, Daniel C., Bomers, Marije K., Cossey, Veerle, Hulzebos, Christian V., van Kaam, Anton H., Kramer, Boris W., van Lingen, Richard A., Wicaksono, Alfian, Covington, James A., van Weissenbruch, Mirjam M., Benninga, Marc A., de Boer, Nanne K. H. and de Meij, Tim G. J. (2020) Preclinical detection of non-catheter related late-onset sepsis in preterm infants by fecal volatile compounds analysis : a prospective, multi-center cohort study. The Pediatric Infectious Disease Journal . doi:10.1097/INF.0000000000002589.
Access rights to Published version:	Restricted or Subscription Access

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