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Data for Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR
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Simmons, Thomas J., Mortimer, Jenny C., Bernardinelli, Oigres D., Pöppler, Ann Christin, Brown, Steven P., deAzevedo, Eduardo R., Dupree, Ray and Dupree, Paul (2016) Data for Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR. [Dataset]
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Official URL: https://wrap.warwick.ac.uk/134766
Abstract
Exploitation of plant lignocellulosic biomass is hampered by our ignorance of the molecular basis for its properties such as strength and digestibility. Xylan, the most prevalent non-cellulosic polysaccharide, binds to cellulose microfibrils. The nature of this interaction remains unclear, despite its importance. Here we show that the majority of xylan, which forms a threefold helical screw in solution, flattens into a twofold helical screw ribbon to bind intimately to cellulose microfibrils in the cell wall. 13C solid-state magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy, supported by in silico predictions of chemical shifts, shows both two- and threefold screw xylan conformations are present in fresh Arabidopsis stems. The twofold screw xylan is spatially close to cellulose, and has similar rigidity to the cellulose microfibrils, but reverts to the threefold screw conformation in the cellulose-deficient irx3 mutant. The discovery that induced polysaccharide conformation underlies cell wall assembly provides new principles to understand biomass properties.
Item Type: | Dataset | |||||||||||||||||||||||||||
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Subjects: | Q Science > QK Botany | |||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | |||||||||||||||||||||||||||
Type of Data: | Experimental data | |||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Xylans, Polysaccharides, Microfibrils, Arabidopsis | |||||||||||||||||||||||||||
Publisher: | University of Warwick, Department of Physics | |||||||||||||||||||||||||||
Official Date: | 12 October 2016 | |||||||||||||||||||||||||||
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Status: | Not Peer Reviewed | |||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||
Media of Output (format): | .m .c | |||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
Copyright Holders: | University of Warwick | |||||||||||||||||||||||||||
Description: | Data record consists of two zip archives containing the data and the software for opening the data respectively. |
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