Pires da Silva, André (2017) Data for Sex- and gamete-specific patterns of X chromosome segregation in a trioecious nematode. [Dataset]

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Abstract

Three key steps in meiosis allow diploid organisms to produce haploid gametes: (1) homologous chromosomes (homologs) pair and undergo crossovers; (2) homologs segregate to opposite poles; and (3) sister chromatids segregate to opposite poles. The XX/XO sex determination system found in many nematodes [1] facilitates the study of meiosis because variation is easily recognized [2, 3, 4]. Here we show that meiotic segregation of X chromosomes in the trioecious nematode Auanema rhodensis [5] varies according to sex (hermaphrodite, female, or male) and type of gametogenesis (oogenesis or spermatogenesis). In this species, XO males exclusively produce X-bearing sperm [6, 7]. The unpaired X precociously separates into sister chromatids, which co-segregate with the autosome set to generate a functional haplo-X sperm. The other set of autosomes is discarded into a residual body. Here we explore the X chromosome behavior in female and hermaphrodite meioses. Whereas X chromosomes segregate following the canonical pattern during XX female oogenesis to yield haplo-X oocytes, during XX hermaphrodite oogenesis they segregate to the first polar body to yield nullo-X oocytes. Thus, crosses between XX hermaphrodites and males yield exclusively male progeny. During hermaphrodite spermatogenesis, the sister chromatids of the X chromosomes separate during meiosis I, and homologous X chromatids segregate to the functional sperm to create diplo-X sperm. Given these intra-species, intra-individual, and intra-gametogenesis variations in the meiotic program, A. rhodensis is an ideal model for studying the plasticity of meiosis and how it can be modulated.

Item Type:	Dataset
Divisions:	Faculty of Science > Life Sciences (2010- )
Type of Data:	Experimental data
Publisher:	Mendeley
Official Date:	14 November 2017
Dates:	Date Event 14 November 2017 Published 14 November 2017 Updated 22 December 2017 Updated
Status:	Not Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Copyright Holders:	University of Warwick
Description:	Data record consists of a single data file in .xlsx format. This excel document contains information for the genotyping of 5 X chromosome markers and 5 autosomal (LG4) markers of the nematode Auanema rhodensis. Each marker contains a Single Nucleotide Polymorphism (SNP) between two strains of A. rhodensis (APS4 and APS6). This SNP creates a restriction enzyme cut site in one strain but not the other. Genotyping of the markers is performed by PCR amplification followed by digestion with a restriction enzyme. This spread sheet includes, for each marker, the full marker sequence, the position and nucleotide difference characterizing the SNP, the forward and reverse primers used to amplify the marker, the restriction enzyme used to differentiate one strain from the other, the strain being cut and the undigested and digested PCR product sizes.
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID BB/L019884/1 [BBSRC] Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 201116/2014-6 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior http://dx.doi.org/10.13039/501100002322 201116/2014-6 Conselho Nacional de Desenvolvimento Científico e Tecnológico http://dx.doi.org/10.13039/501100003593 IOS 1122095 National Science Foundation http://dx.doi.org/10.13039/501100008982 IOS 1122101 National Science Foundation http://dx.doi.org/10.13039/501100008982
Related URLs:	Related item in WRAP Other
Contributors:	Contribution Name Contributor ID Contact Person Pires da Silva, André 55767

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