Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Defective RNAs of Semliki Forest virus

Tools
- Tools
+ Tools

Thomson, Michael (1993) Defective RNAs of Semliki Forest virus. PhD thesis, University of Warwick.

[img]
Preview
PDF
WRAP_Theses_Thomson_1993.pdf - Unspecified Version - Requires a PDF viewer.

Download (4Mb) | Preview
Official URL: http://webcat.warwick.ac.uk/record=b1449336~S15

Request Changes to record.

Abstract

This thesis describes an investigation into the mouse-protecting nucleotide sequences of defective interfering (Dl) Semliki Forest virus (SFV) RNA was extracted from tissue culture preparations of Dl SFV and reverse transcribed Putative Dl SFV cDNA was amplified by the polymerase chain reaction using primers specific for the termini of the virion RNA A number of molecular clones were constructed from the products of amplification and the nucleotide sequences of two of these clones were determined (pSFVDI-6 2146 nts and pSFVDI-19 1244 nts) Both pSFVDI-6 and pSFVDI-19 were derived from three noncontiguous regions of the SFV genome comprising the 5' and 3' termini and part of the nsP2 coding region RNA transcribed from these clones was transfected into SFV-infected BHK-21 cells to produce genetically homogeneous Dl SFV preparations These preparations were stable on serial passage and interfered with virus multiplication m vitro The transfection technique was also used in a preliminary investigation of the regulatory elements of the SFV genome A 388- nucleotide region within the nsP2 gene of SFV was tentatively defined as containing all or part of a packaging signal since Dl SFV clones lacking this region were not propagated as virions.

To determine the biological activity of the cloned Dl SFV preparations in vivo they were mixed with 10 LD₁₀, SFV and inoculated into adult mice by the intranasal route The Dl SFV preparation derived from pSFVDI-19 typically conferred 75% protection against the lethal encephalitis that normally follows infection with SFV. whereas the Dl SFV preparation derived from pSFVDI-6 was non-protecting However, it should be noted that the concentration of Dl SFV in these cloned preparations was not standardised Modulation of infection in vivo was independent of the antigenic load and mice were susceptible to subsequent lethal challenge A preliminary experiment suggested that propagation of Dl SFV genomes was cell-specific because genomes derived from pSFVDI-19, but not pSFVDI-6, could be detected in mouse brain tissue following intracerebral coinoculation of SFV with the cloned Dl SFV preparations.

Item Type: Thesis or Dissertation (PhD)
Subjects: Q Science > QP Physiology
Library of Congress Subject Headings (LCSH): Semliki Forest virus, RNA, Nucleotides
Official Date: April 1993
Dates:
DateEvent
April 1993UNSPECIFIED
Institution: University of Warwick
Theses Department: Department of Biological Sciences
Thesis Type: PhD
Publication Status: Unpublished
Supervisor(s)/Advisor: Dimmock, N. J.
Sponsors: Medical Research Council (Great Britain)
Extent: xvi, 245 leaves : illustrations
Language: eng

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us