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A Python based automated tracking routine for myosin II filaments

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Mosby, Lewis S., Polin, Marco and Köster, Darius Vasco (2020) A Python based automated tracking routine for myosin II filaments. Journal of Physics D: Applied Physics, 53 (30). 304002. doi:10.1088/1361-6463/ab87bf

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Official URL: http://dx.doi.org/10.1088/1361-6463/ab87bf

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Abstract

The study of motor protein dynamics within cytoskeletal networks is of high interest to physicists and biologists to understand how the dynamics and properties of individual motors lead to cooperative effects and control of overall network behaviour. Here, we report a method to detect and track muscular myosin II filaments within an actin network tethered to supported lipid bilayers. Based on the characteristic shape of myosin II filaments, this automated tracking routine allowed us to follow the position and orientation of myosin II filaments over time, and to reliably classify their dynamics into segments of diffusive and processive motion based on the analysis of displacements and angular changes between time steps. This automated, high throughput method will allow scientists to efficiently analyse motor dynamics in different conditions, and will grant access to more detailed information than provided by common tracking methods, without any need for time consuming manual tracking or generation of kymographs.

Item Type: Journal Article
Subjects: Q Science > QC Physics
Q Science > QP Physiology
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Science > Physics
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Particle tracks (Nuclear physics), Myosin, Cytoskeleton -- Microbiology
Journal or Publication Title: Journal of Physics D: Applied Physics
Publisher: IOP Publishing
ISSN: 0022-3727
Official Date: 27 May 2020
Dates:
DateEvent
27 May 2020Published
8 April 2020Available
8 April 2020Accepted
Date of first compliant deposit: 9 April 2020
Volume: 53
Number: 30
Article Number: 304002
DOI: 10.1088/1361-6463/ab87bf
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
RMRCB0058Wellcome Trusthttp://dx.doi.org/10.13039/100010269
RPG-2016-260Leverhulme Trusthttp://dx.doi.org/10.13039/501100000275

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