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Cell size control driven by the circadian clock and environment in cyanobacteria
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Martins, Bruno M. C., Tooke, Amy K., Thomas, Philipp and Locke, James C. W. (2018) Cell size control driven by the circadian clock and environment in cyanobacteria. Proceedings of the National Academy of Sciences of the United States of America, 115 (48). E11415-E11424. doi:10.1073/pnas.1811309115 ISSN 0027-8424.
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Official URL: http://dx.doi.org/10.1073/pnas.1811309115
Abstract
How cells maintain their size has been extensively studied under constant conditions. In the wild, however, cells rarely experience constant environments. Here, we examine how the 24-h circadian clock and environmental cycles modulate cell size control and division timings in the cyanobacterium Synechococcus elongatus using single-cell time-lapse microscopy. Under constant light, wild-type cells follow an apparent sizer-like principle. Closer inspection reveals that the clock generates two subpopulations, with cells born in the subjective day following different division rules from cells born in subjective night. A stochastic model explains how this behavior emerges from the interaction of cell size control with the clock. We demonstrate that the clock continuously modulates the probability of cell division throughout day and night, rather than solely applying an on−off gate to division, as previously proposed. Iterating between modeling and experiments, we go on to identify an effective coupling of the division rate to time of day through the combined effects of the environment and the clock on cell division. Under naturally graded light−dark cycles, this coupling narrows the time window of cell divisions and shifts divisions away from when light levels are low and cell growth is reduced. Our analysis allows us to disentangle, and predict the effects of, the complex interactions between the environment, clock, and cell size control.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||||
Publisher: | National Academy of Sciences | ||||||
ISSN: | 0027-8424 | ||||||
Official Date: | 27 November 2018 | ||||||
Dates: |
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Volume: | 115 | ||||||
Number: | 48 | ||||||
Page Range: | E11415-E11424 | ||||||
DOI: | 10.1073/pnas.1811309115 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) |
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