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Investigating bacteriophages targeting the opportunistic pathogen Acinetobacter baumannii

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Styles, Kathryn, Thummeepak, Rapee, Leungtongkam, Udomluk, Smith, Sophie, Christie, Gabrielle S., Millard, Andrew D., Moat, John, Dowson, Christopher G., Wellington, Elizabeth M. H., Sitthisak, Sutthirat and Sagona, Antonia P. (2020) Investigating bacteriophages targeting the opportunistic pathogen Acinetobacter baumannii. Antibiotics, 9 (4). 200. doi:10.3390/antibiotics9040200

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Official URL: https://doi.org/10.3390/antibiotics9040200

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Abstract

The multi-drug resistance of the opportunistic pathogen Acinetobacter baumannii is of growing concern, with many clinical isolates proving to be resistant to last resort as well as front line antibiotic treatments. The use of bacteriophages is an attractive alternative to controlling and treating this emerging nosocomial pathogen. In this study, we have investigated bacteriophages collected from hospital wastewater in Thailand and we have explored their activity against clinical isolates of A. baumannii. Bacteriophage vB_AbaM_PhT2 showed 28% host range against 150 multidrug resistant (MDR) isolates and whole genome sequencing did not detect any known virulence factors or antibiotic resistance genes. Purified vB_AbaM_PhT2 samples had endotoxin levels below those recommended for preclinical trials and were not shown to be directly cytotoxic to human cell lines in vitro. The treatment of human brain and bladder cell lines grown in the presence of A. baumannii with this bacteriophage released significantly less lactate dehydrogenase compared to samples with no bacteriophage treatment, indicating that vB_AbaM_PhT2 can protect from A. baumannii induced cellular damage. Our results have also indicated that there is synergy between this bacteriophage and the end line antibiotic colistin. We therefore propose bacteriophage vB_AbaM_PhT2 as a good candidate for future research and for its potential development into a surface antimicrobial for use in hospitals. View Full-Text

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Acinetobacter infections, Drug resistance in microorganisms, Anti-infective agents, Anti-infective agents -- Thailand, Bacteriophages
Journal or Publication Title: Antibiotics
Publisher: M.D.P.I.
ISSN: 2079-6382
Official Date: 22 April 2020
Dates:
DateEvent
22 April 2020Published
20 April 2020Accepted
Date of first compliant deposit: 22 April 2020
Volume: 9
Number: 4
Article Number: 200
DOI: 10.3390/antibiotics9040200
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
332371796Department for Business, Energy and Industrial Strategyhttp://dx.doi.org/10.13039/100011693
UNSPECIFIEDBritish Councilhttp://dx.doi.org/10.13039/501100000308
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