Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Molecular analysis of the pmo (particulate methane monooxygenase) operons from two type II methanotrophs

Tools
- Tools
+ Tools

UNSPECIFIED (2000) Molecular analysis of the pmo (particulate methane monooxygenase) operons from two type II methanotrophs. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66 (3). pp. 966-975.

Full text not available from this repository, contact author.

Request Changes to record.

Abstract

The particulate methane monooxygenase gene clusters, pmoCAB, from two representative type II methanotrophs of the alpha-Proteobacteria, Methylosinus trichosporium OB3b and Methylocystis sp, strain M, have been cloned and sequenced. Primer extension experiments revealed that the pmo cluster is probably transcribed from a single transcriptional start site located 300 bp upstream of the start of the first gene, pmoC, for Methylocystis sp, strain M, Immediately upstream of the putative start site, consensus sequences for sigma(70) promoters were identified, suggesting that these pmo genes are recognized by sigma(70) and negatively regulated under low-copper conditions. The pmo genes were cloned in several overlapping fragments, since parts of these genes appeared to be toxic to the Escherichia coil host. Methanotrophs contain two virtually identical copies of pmo genes, and it was necessary to use Southern blotting and probing with pmo gene fragments in order to differentiate between the two pmoCAB clusters in both methanotrophs. The complete DNA sequence of one copy of pmo genes from each organism is reported here. The gene sequences are 84% similar to each other and 75% similar to that of a type I methanotroph of the gamma-Proteobacteria, Methylococcus capsulatus Bath. The derived proteins PmoC and PmoA are predicted to be highly hydrophobic and consist mainly of transmembrane-spanning regions, whereas PmoB has only two putative transmembrane-spanning helices, Hybridization experiments showed that there are two copies of pmoC in both M, trichosporium OB3b and Methylocystis sp, strain M, and not three copies as found in M. capsulatus Bath.

Item Type: Journal Article
Subjects: T Technology > TP Chemical technology
Q Science > QR Microbiology
Journal or Publication Title: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Publisher: AMER SOC MICROBIOLOGY
ISSN: 0099-2240
Official Date: March 2000
Dates:
DateEvent
March 2000UNSPECIFIED
Volume: 66
Number: 3
Number of Pages: 10
Page Range: pp. 966-975
Publication Status: Published

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: publications@live.warwick.ac.uk
Contact Details
About Us