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Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
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Osborn, Daniel P. S., Li, Kuoyu, Cutty, Stephen J., Nelson, A. C. (Andrew Christopher), Wardle, Fiona C., Hinits, Yaniv and Hughes, Simon M. (2020) Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis. Development , 147 (8). dev184689. doi:10.1242/dev.184689
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WRAP-Fgf-driven-Tbx-protein-induce-myf5-myod-Nelson-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (15Mb) | Preview |
Official URL: http://dx.doi.org/10.1242/dev.184689
Abstract
Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived Fgf signals act through Tbx16 and Tbxta to induce slow and fast trunk muscle precursors at distinct dorsoventral positions. Tbx16 binds to and directly activates the myf5 and myod genes, which are required for commitment to myogenesis. Tbx16 activity depends on Fgf signalling from the organiser. In contrast, Tbxta is not required for myf5 expression, but binds a specific site upstream of myod that is not bound by Tbx16 and drives (dependent on Fgf signals) myod expression in adaxial slow precursors, thereby initiating trunk myogenesis. After gastrulation, when similar muscle cell populations in the post-anal tail are generated from tailbud, declining Fgf signalling is less effective at initiating adaxial myogenesis, which is instead initiated by Hedgehog signalling from the notochord. Our findings suggest a hypothesis for ancestral vertebrate trunk myogenic patterning and how it was co-opted during tail evolution to generate similar muscle by new mechanisms.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QL Zoology Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Logperch , Myogenesis, Skeleton -- Muscles, Myosin, Fibroblast growth factors | |||||||||||||||
| Journal or Publication Title: | Development | |||||||||||||||
| Publisher: | The Company of Biologists Ltd. | |||||||||||||||
| ISSN: | 0950-1991 | |||||||||||||||
| Official Date: | 28 April 2020 | |||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 11 May 2020 | |||||||||||||||
| Volume: | 147 | |||||||||||||||
| Number: | 8 | |||||||||||||||
| Article Number: | dev184689 | |||||||||||||||
| DOI: | 10.1242/dev.184689 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||
| Copyright Holders: | © 2020. Published by The Company of Biologists Ltd | |||||||||||||||
| RIOXX Funder/Project Grant: |
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