Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis

Tools
- Tools
+ Tools

Osborn, Daniel P. S., Li, Kuoyu, Cutty, Stephen J., Nelson, A. C. (Andrew Christopher), Wardle, Fiona C., Hinits, Yaniv and Hughes, Simon M. (2020) Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis. Development , 147 (8). dev184689. doi:10.1242/dev.184689

[img]
Preview
PDF
WRAP-Fgf-driven-Tbx-protein-induce-myf5-myod-Nelson-2020.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (15Mb) | Preview
Official URL: http://dx.doi.org/10.1242/dev.184689

Request Changes to record.

Abstract

Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived Fgf signals act through Tbx16 and Tbxta to induce slow and fast trunk muscle precursors at distinct dorsoventral positions. Tbx16 binds to and directly activates the myf5 and myod genes, which are required for commitment to myogenesis. Tbx16 activity depends on Fgf signalling from the organiser. In contrast, Tbxta is not required for myf5 expression, but binds a specific site upstream of myod that is not bound by Tbx16 and drives (dependent on Fgf signals) myod expression in adaxial slow precursors, thereby initiating trunk myogenesis. After gastrulation, when similar muscle cell populations in the post-anal tail are generated from tailbud, declining Fgf signalling is less effective at initiating adaxial myogenesis, which is instead initiated by Hedgehog signalling from the notochord. Our findings suggest a hypothesis for ancestral vertebrate trunk myogenic patterning and how it was co-opted during tail evolution to generate similar muscle by new mechanisms.

Item Type: Journal Article
Subjects: Q Science > QL Zoology
Q Science > QP Physiology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Logperch , Myogenesis, Skeleton -- Muscles, Myosin, Fibroblast growth factors
Journal or Publication Title: Development
Publisher: The Company of Biologists Ltd.
ISSN: 0950-1991
Official Date: 28 April 2020
Dates:
DateEvent
28 April 2020Published
UNSPECIFIEDAvailable
14 February 2020Accepted
Date of first compliant deposit: 11 May 2020
Volume: 147
Number: 8
Article Number: dev184689
DOI: 10.1242/dev.184689
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Copyright Holders: © 2020. Published by The Company of Biologists Ltd
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
G1001029Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
MR/N021231/1Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
PG/14/12/30664British Heart Foundationhttp://dx.doi.org/10.13039/501100000274
PG/13/19/30059British Heart Foundationhttp://dx.doi.org/10.13039/501100000274

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us