UNSPECIFIED. (2000) Biochemical and physical characterization of parvovirus minute virus of mice virus-like particles. VIROLOGY, 267 (2). pp. 299-309. ISSN 0042-6822

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Abstract

The VP-2 major capsid protein of the prototype strain of the parvovirus minute virus of mice (MVMp) was expressed, using a baculovirus vector, in Sf9 insect cells. Immunogold electron microscopy of infected Sf9 cells showed VP-2 localized in the nucleus and cytoplasm as is observed in mammalian cells during natural infections. The VP-2 subunits self-assembled into empty parvovirus-like particles (VLPs), which appeared morphologically similar to and immunogenically indistinguishable from native empty MVMp particles, which also contain the minor capsid protein, VP1. Incubations under different pH and temperature conditions showed that the MVMp VLPs and native empty MVMp capsids share comparable stability. Once heated the particles can be similarly and specifically cleaved by trypsin at the VP-2 N-terminal domain. This process mimics the further maturation of the "rat-like" parvovirus virions, following viral DNA encapsidation, indicating that biologically relevant features of the MVMp capsid are maintained in the VLPs. Crystals have been obtained for the MVMp VLPs which were isomorphous to those used for the high-resolution structure determination of virions and native empty particles of the immunosuppressive strain of MVM (MVMI). The VLP crystals diffracted X rays to beyond 3-Angstrom resolution and are in space group C2 (a = 448.7, b = 416.6, c = 306.1 Angstrom, and beta = 95.9 Angstrom). This is the first report of crystals from parvoviral particles produced in a heterologous system diffracting X rays to high resolution, indicating that VP-2 of some parvovirus capsids can self-assemble into ordered T = 1 icosahedral capsids in the absence of other viral and host cell functions. (C) 2000 Academic Press.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	VIROLOGY
Publisher:	ACADEMIC PRESS INC
ISSN:	0042-6822
Date:	15 February 2000
Volume:	267
Number:	2
Number of Pages:	11
Page Range:	pp. 299-309
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/13674

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