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Nanoscale tweezers for single-cell biopsies
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Nadappuram, Binoy Paulose, Cadinu, Paolo, Barik, Avijit, Ainscough, Alexander J., Devine, Michael J., Kang, Minkyung, Gonzalez-Garcia, Jorge, Kittler, Josef T., Willison, Keith R. , Vilar, Ramon, Actis, Paolo, Wojciak-Stothard, Beata, Oh, Sang-Hyun, Ivanov, Aleksandar P and Edel, Joshua B. (2019) Nanoscale tweezers for single-cell biopsies. Nature Nanotechnology, 14 . pp. 80-88. doi:10.1038/s41565-018-0315-8 ISSN 1748-3387.
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Official URL: https://doi.org/10.1038/s41565-018-0315-8
Abstract
Much of the functionality of multicellular systems arises from the spatial organization and dynamic behaviours within and between cells. Current single-cell genomic methods only provide a transcriptional ‘snapshot’ of individual cells. The real-time analysis and perturbation of living cells would generate a step change in single-cell analysis. Here we describe minimally invasive nanotweezers that can be spatially controlled to extract samples from living cells with single-molecule precision. They consist of two closely spaced electrodes with gaps as small as 10–20 nm, which can be used for the dielectrophoretic trapping of DNA and proteins. Aside from trapping single molecules, we also extract nucleic acids for gene expression analysis from living cells without affecting their viability. Finally, we report on the trapping and extraction of a single mitochondrion. This work bridges the gap between single-molecule/organelle manipulation and cell biology and can ultimately enable a better understanding of living cells.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Scanning probe microscopy, Ion channels, Gene expression, Cytology -- Technique, Cells -- Analysis | ||||||||||||||||||
Journal or Publication Title: | Nature Nanotechnology | ||||||||||||||||||
Publisher: | Springer Nature | ||||||||||||||||||
ISSN: | 1748-3387 | ||||||||||||||||||
Official Date: | 31 January 2019 | ||||||||||||||||||
Dates: |
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Volume: | 14 | ||||||||||||||||||
Page Range: | pp. 80-88 | ||||||||||||||||||
DOI: | 10.1038/s41565-018-0315-8 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||
Date of first compliant deposit: | 22 May 2020 | ||||||||||||||||||
Date of first compliant Open Access: | 22 May 2020 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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Open Access Version: |
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