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Novel markers for high-throughput protoplast-based analyses of phytohormone signaling
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Lehmann, Silke, Dominguez-Ferreras, Ana, Huang, Wei-Jie, Denby, Katherine J., Ntoukakis, Vardis and Schäfer, Patrick (2020) Novel markers for high-throughput protoplast-based analyses of phytohormone signaling. PLoS One, 15 (6). e0234154. doi:10.1371/journal.pone.0234154 ISSN 1932-6203.
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Official URL: https://doi.org/10.1371/journal.pone.0234154
Abstract
Phytohormones mediate most diverse processes in plants, ranging from organ development to immune responses. Receptor protein complexes perceive changes in intracellular phytohormone levels and trigger a signaling cascade to effectuate downstream responses. The in planta analysis of elements involved in phytohormone signaling can be achieved through transient expression in mesophyll protoplasts, which are a fast and versatile alternative to generating plant lines that stably express a transgene. While promoter-reporter constructs have been used successfully to identify internal or external factors that change phytohormone signaling, the range of available marker constructs does not meet the potential of the protoplast technique for large scale approaches. The aim of our study was to provide novel markers for phytohormone signaling in the Arabidopsis mesophyll protoplast system. We validated 18 promoter::luciferase constructs towards their phytohormone responsiveness and specificity and suggest an experimental setup for high-throughput analyses. We recommend novel markers for the analysis of auxin, abscisic acid, cytokinin, salicylic acid and jasmonic acid responses that will facilitate future screens for biological elements and environmental stimuli affecting phytohormone signaling.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QK Botany | |||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
| Library of Congress Subject Headings (LCSH): | Plant hormones -- Research, Growth (Plants), Plant cellular signal transduction, Plant molecular biology | |||||||||
| Journal or Publication Title: | PLoS One | |||||||||
| Publisher: | Public Library of Science | |||||||||
| ISSN: | 1932-6203 | |||||||||
| Official Date: | 4 June 2020 | |||||||||
| Dates: |
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| Volume: | 15 | |||||||||
| Number: | 6 | |||||||||
| Article Number: | e0234154 | |||||||||
| DOI: | 10.1371/journal.pone.0234154 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 27 May 2020 | |||||||||
| Date of first compliant Open Access: | 10 June 2020 | |||||||||
| RIOXX Funder/Project Grant: |
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