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Fluorescence lifetime imaging reveals regulation of presynaptic Ca+ by glutamate uptake and mGluRs, but not somatic voltage in cortical neurons
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Tyurikova, Olga, Zheng, Kaiyu, Nicholson, Elizabeth, Timofeeva, Yulia, Semyanov, Alexey, Volynski, Kirill and Rusakov, Dmitri A. (2021) Fluorescence lifetime imaging reveals regulation of presynaptic Ca+ by glutamate uptake and mGluRs, but not somatic voltage in cortical neurons. Journal of Neurochemistry, 156 (1). pp. 48-58. doi:10.1111/jnc.15094 ISSN 0022-3042.
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Official URL: https://doi.org/10.1111/jnc.15094
Abstract
Brain function relies on vesicular release of neurotransmitters at chemical synapses. The release probability depends on action potential-evoked presynaptic Ca entry, but also on the resting Ca level. Whether these basic aspects of presynaptic calcium homeostasis show any consistent trend along the axonal path, and how they are controlled by local network activity, remains poorly understood. Here, we take advantage of the recently advanced FLIM-based method to monitor presynaptic Ca with nanomolar sensitivity. We find that, in cortical pyramidal neurons, action potential- evoked calcium entry (range 10-300 nM), but not the resting Ca level (range 10-100 nM), tends to increase with higher order of axonal branches. Blocking astroglial glutamate uptake reduces evoked Ca entry but has little effect on resting Ca whereas both appear boosted by the constitutive activation of group 1/2 metabotropic glutamate receptors. We find no consistent effect of transient somatic depolarisation or hyperpolarisation on presynaptic Ca entry or its basal level. The results unveil some key aspects of presynaptic machinery in cortical circuits, shedding light on basic principles of synaptic connectivity in the brain. [Abstract copyright: This article is protected by copyright. All rights reserved.]
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Computer Science | ||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Fluorescence spectroscopy, Neurosciences -- Data processing, Glutamic acid -- Receptors, Cerebral cortex -- Research, Brain, Neurotransmitter receptors, G proteins -- Receptors | ||||||||||||||||||
Journal or Publication Title: | Journal of Neurochemistry | ||||||||||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||||||||
ISSN: | 0022-3042 | ||||||||||||||||||
Official Date: | January 2021 | ||||||||||||||||||
Dates: |
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Volume: | 156 | ||||||||||||||||||
Number: | 1 | ||||||||||||||||||
Page Range: | pp. 48-58 | ||||||||||||||||||
DOI: | 10.1111/jnc.15094 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Reuse Statement (publisher, data, author rights): | "This is the peer reviewed version of the following article: Tyurikova, O., Zheng, K., Nicholson, E., Timofeeva, Y., Semyanov, A., Volynski, K. and Rusakov, D.A. (2020), Fluorescence lifetime imaging reveals regulation of presynaptic Ca2+ by glutamate uptake and mGluRs, but not somatic voltage in cortical neurons. J. Neurochem.. Accepted Author Manuscript. which has been published in final form atdoi:10.1111/jnc.15094, This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Description: | Free access |
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Date of first compliant deposit: | 1 June 2020 | ||||||||||||||||||
Date of first compliant Open Access: | 17 May 2021 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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