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Meiosis associated Argonautes in male reproductive development
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Maple, Robert (2019) Meiosis associated Argonautes in male reproductive development. PhD thesis, University of Warwick.
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WRAP_Theses_Maple_2019.pdf - Submitted Version - Requires a PDF viewer. Download (32Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3452936~S15
Abstract
Unlike animals, plants do not specify their germline until late in development from adult somatic cells. During the specification of the germline, dynamic epigenetic reprogramming events take place, necessary to reset somatic epigenetic marks. During this stage, the plant germline is especially susceptible to transposable element activation and transposition events. Therefore, plants must deploy diverse mechanisms to tightly control the transcription and translation of these repetitive elements. Argonaute proteins are thought to be critical components of the RNA mediated epigenetic silencing involved in these processes. However, their precise mechanisms in reproductive development are not known. We identified two meiosis associated Argonautes that accumulate in developing anthers and bind to a discrete class of sRNA. Genetic analysis revealed that these proteins are necessary for male reproductive development and plants defective in MAGO proteins display male fertility defects, especially when exposed to heat stress. Genomic analysis revealed that MAGO proteins regulate the transcriptional activation of LTR retrotransposons after heat stress. Collectively, our data indicates that MAGO and associated small RNAs are required to maintain the male germline under stress.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
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Library of Congress Subject Headings (LCSH): | Meiosis, RNA interference, Gene silencing, Germ cells | ||||
Official Date: | August 2019 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Gutierrez-Marcos, José F. | ||||
Sponsors: | Biogemma ; Biotechnology and Biological Sciences Research Council (Great Britain) | ||||
Format of File: | |||||
Extent: | xiv, 226 leaves : illustrations | ||||
Language: | eng |
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