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Microsatellite marker development in the crop wild relative Linum bienne using genome skimming
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Landoni, Beatrice, Viruel, Juan, Gómez, Rocio, Allaby, Robin G., Brennan, Adrian C., Picó, F. Xavier and Pérez‐Barrales, Rocio (2020) Microsatellite marker development in the crop wild relative Linum bienne using genome skimming. Applications in Plant Sciences, 8 (5). e11349. doi:10.1002/aps3.11349 ISSN 2168-0450.
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Official URL: https://doi.org/10.1002/aps3.11349
Abstract
Nuclear microsatellite markers were developed for Linum bienne , the sister species of the crop L. usitatissimum , to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure.
Methods and Results
Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross‐amplified in L. usitatissimum .
Conclusions
The 16 microsatellite loci developed here can be used for population genetic studies in L. bienne , and 28 additional loci that successfully amplified are available for further testing.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QK Botany |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||
Library of Congress Subject Headings (LCSH): | Plants, Linaceae, Population genetics, Microsatellites (Genetics) | ||||||||||||
Journal or Publication Title: | Applications in Plant Sciences | ||||||||||||
Publisher: | Wiley | ||||||||||||
ISSN: | 2168-0450 | ||||||||||||
Official Date: | 26 May 2020 | ||||||||||||
Dates: |
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Volume: | 8 | ||||||||||||
Number: | 5 | ||||||||||||
Article Number: | e11349 | ||||||||||||
DOI: | 10.1002/aps3.11349 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 18 June 2020 | ||||||||||||
Date of first compliant Open Access: | 18 June 2020 | ||||||||||||
RIOXX Funder/Project Grant: |
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