The Library
Building a better biofilm - formation of in vivo-like biofilm structures by Pseudomonas aeruginosa in a porcine model of cystic fibrosis lung infection
Tools
Harrington, Niamh, Sweeney, Esther and Harrison, Freya (2020) Building a better biofilm - formation of in vivo-like biofilm structures by Pseudomonas aeruginosa in a porcine model of cystic fibrosis lung infection. Biofilm, 2 . 100024. doi:10.1016/j.bioflm.2020.100024 ISSN 2590-2075.
|
PDF
WRAP-Building-biofilm-formation-porcine-model-fibrosis-lung-Harrison-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (2511Kb) | Preview |
Official URL: https://doi.org/10.1016/j.bioflm.2020.100024
Abstract
Pseudomonas aeruginosa biofilm infections in the cystic fibrosis (CF) lung are highly resistant to current antimicrobial treatments and are associated with increased mortality rates. The existing models for such infections are not able to reliably mimic the clinical biofilms observed. We aimed to further optimise an ex vivo pig lung (EVPL) model for P. aeruginosa CF lung infection that can be used to increase understanding of chronic CF biofilm infection. The EVPL model will facilitate discovery of novel infection prevention methods and treatments, and enhanced exploration of biofilm architecture. We investigated purine metabolism and biofilm formation in the model using transposon insertion mutants in P. aeruginosa PA14 for key genes: purD, gacA and pelA. Our results demonstrate that EVPL recapitulates a key aspect of in vivo P. aeruginosa infection metabolism, and that the pathogen forms a biofilm with a clinically realistic structure not seen in other in vitro studies. Two pathways known to be required for in vivo biofilm infection - the Gac regulatory pathway and production of the Pel exopolysaccharide - are essential to the formation of this mature, structured biofilm on EVPL tissue. We propose the high-throughput EVPL model as a validated biofilm platform to bridge the gap between in vitro work and CF lung infection.
Item Type: | Journal Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology R Medicine > RC Internal medicine |
|||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
SWORD Depositor: | Library Publications Router | |||||||||
Library of Congress Subject Headings (LCSH): | Biofilms, Cystic fibrosis , Lungs -- Diseases, Lungs -- Diseases -- Molecular aspects | |||||||||
Journal or Publication Title: | Biofilm | |||||||||
Publisher: | Elsevier | |||||||||
ISSN: | 2590-2075 | |||||||||
Official Date: | December 2020 | |||||||||
Dates: |
|
|||||||||
Volume: | 2 | |||||||||
Article Number: | 100024 | |||||||||
DOI: | 10.1016/j.bioflm.2020.100024 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 15 June 2020 | |||||||||
Date of first compliant Open Access: | 16 June 2020 | |||||||||
RIOXX Funder/Project Grant: |
|
|||||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year