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Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent
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Kamennaya, Nina, Geraki, Kalotina, Scanlan, David J. and Zubkov, Mikhail V. (2020) Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent. Nature Communications, 11 (1). 2642. doi:10.1038/s41467-020-16428-w
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WRAP-Accumulation-ambient-phosphate-periplasm-marine-bacteria-Scanlan-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2310Kb) | Preview |
Official URL: https://doi.org/10.1038/s41467-020-16428-w
Abstract
Bacteria acquire phosphate (P ) by maintaining a periplasmic concentration below environmental levels. We recently described an extracellular P buffer which appears to counteract the gradient required for P diffusion. Here, we demonstrate that various treatments to outer membrane (OM) constituents do not affect the buffered P because bacteria accumulate P in the periplasm, from which it can be removed hypo-osmotically. The periplasmic P can be gradually imported into the cytoplasm by ATP-powered transport, however, the proton motive force (PMF) is not required to keep P in the periplasm. In contrast, the accumulation of P into the periplasm across the OM is PMF-dependent and can be enhanced by light energy. Because the conventional mechanism of P -specific transport cannot explain P accumulation in the periplasm we propose that periplasmic P anions pair with chemiosmotic cations of the PMF and millions of accumulated P pairs could influence the periplasmic osmolarity of marine bacteria.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Marine bacteria, Marine microbiology, Marine chemical ecology, Phosphates -- Absorption and adsorption | |||||||||
| Journal or Publication Title: | Nature Communications | |||||||||
| Publisher: | Nature Publishing Group | |||||||||
| ISSN: | 2041-1723 | |||||||||
| Official Date: | 26 May 2020 | |||||||||
| Dates: |
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| Date of first compliant deposit: | 16 June 2020 | |||||||||
| Volume: | 11 | |||||||||
| Number: | 1 | |||||||||
| Article Number: | 2642 | |||||||||
| DOI: | 10.1038/s41467-020-16428-w | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Publisher Statement: | ** From PubMed via Jisc Publications Router ** History: received 12-02-2019; accepted 24-04-2020. | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| RIOXX Funder/Project Grant: |
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