Griffiths, Edward John (1975) Studies on inhibitor resistances in the yeast Saccharomyces cerevisiae. PhD thesis, University of Warwick.

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Abstract

A series of mutants were selected on their ability to grow in the presence of the inhibitor of oxidative phosphorylation, oligomycin. The work described in this thesis was an attempt to identify the aberrant peptide(s) responsible for an increase in the resistance to oligomycin in the case of A21 and A15 and also to venturicidin and TET in the case of A19. The factor(s) responsible for these phenomena were shown to be located in an ATPase and OSCP depleted membrane fraction. The mitochondrial membranes from both wild type and mutants were shown to be structurally similar by E.S.R. and ³H-NEM labelling. A soluble inhibitor sensitive ATPase (O.S. ATPase) was prepared from both wild type and mutant strains. The enzyme from the mutants studied showed no aberrations during purification or large scale deletions upon analysis by polyacrylamide gel electrophoresis. The enzyme from both wild type and mutant cells was shown to possess a site of action of oligomycin, venturicidin and TET. The solubilised enzymes were shown to have retained the specific resistance phenomena found at the level of SMP in the mutants studied. One of the mutants, A21, was shown to have aberrant kinetics of formation of inhibitor sensitive ATPase after incubation in CAP. Analysis of the mitoribosomal products over a time course after incubation in CAP was made on wild type and A21 cells. Antisera to O.S. ATPase was used to identify the mitoribosomal products associated with O.S. ATPase. The major mitoribosomal product, subunit 9 of the O.S. ATPase, was purified and partially characterised. Problems concerning the solubility of this product precluded further analysis of it. The appendix describes work carried out on a preparative gel electrophoresis apparatus.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QD Chemistry Q Science > QK Botany
Library of Congress Subject Headings (LCSH):	Saccharomyces cerevisiae, Saccharomyces -- Biotechnology, Chemical inhibitors, Phosphorylation
Official Date:	1975
Dates:	Date Event 1975 UNSPECIFIED
Institution:	University of Warwick
Theses Department:	Department of Chemistry
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Griffiths, D. E.
Sponsors:	Medical Research Council (Great Britain)
Extent:	1 volume (various pagings) : illustrations
Language:	eng

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