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Production of substituted styrene bioproducts from lignin and lignocellulose using engineered pseudomonas putida KT2440
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Williamson, James J., Bahrin, Nurfariza, Hardiman, Elizabeth M. and Bugg, Timothy D. H. (2020) Production of substituted styrene bioproducts from lignin and lignocellulose using engineered pseudomonas putida KT2440. Biotechnology Journal . e1900571. doi:10.1002/biot.201900571 (In Press)
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WRAP-Production-substituted-styrene-bioproducts-lignin-lignocellulose-2020.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only until 2 June 2021. Contact author directly, specifying your specific needs. - Requires a PDF viewer. Download (436Kb) |
Official URL: https://doi.org/10.1002/biot.201900571
Abstract
Ferulic acid is a renewable chemical found in lignocellulose from grasses such as wheat straw and sugarcane. Pseudomonas putida is able to liberate and metabolize ferulic acid from plant biomass. Deletion of the hydroxycinnamoyl-CoA hydratase-lyase gene (ech) produced a strain of P. putida unable to utilize ferulic and p-coumaric acid, which is able to accumulate ferulic acid and p-coumaric acid from wheat straw or sugar cane bagasse. Further engineering of this strain saw the replacement of ech with the phenolic acid decarboxylase padC, which converts p-coumaric and ferulic acid into 4-vinylphenol and the flavor agent 4-vinylguaiacol, respectively. The engineered strain containing padC is able to generate 4-vinylguaiacol and 4-vinylphenol from media containing lignocellulose or Green Value Protobind lignin as feedstock, and does not require the addition of an exogenous inducer molecule. Biopolymerization of 4-vinylguaiacol and 4-vinylcatechol styrene products is also carried out, using Trametes versicolor laccase, to generate "biopolystyrene" materials on small scale. [Abstract copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.]
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Chemistry | ||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Pseudomonas, Metabolism, Hydroxycitric acid, Biological products | ||||||||||||
| Journal or Publication Title: | Biotechnology Journal | ||||||||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||
| ISSN: | 1860-6768 | ||||||||||||
| Official Date: | 2020 | ||||||||||||
| Dates: |
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| Date of first compliant deposit: | 18 June 2020 | ||||||||||||
| Article Number: | e1900571 | ||||||||||||
| DOI: | 10.1002/biot.201900571 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | In Press | ||||||||||||
| Publisher Statement: | "This is the peer reviewed version of the following article: Williamson, J. J., Bahrin, N., Hardiman, E. M., Bugg, T. D. H., Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440. Biotechnol. J. 2020, 1900571. https://doi.org/10.1002/biot.201900571, which has been published in final form at https://doi.org/10.1002/biot.201900571 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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