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Production of substituted styrene bioproducts from lignin and lignocellulose using engineered pseudomonas putida KT2440
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Williamson, James J., Bahrin, Nurfariza, Hardiman, Elizabeth M. and Bugg, Timothy D. H. (2020) Production of substituted styrene bioproducts from lignin and lignocellulose using engineered pseudomonas putida KT2440. Biotechnology Journal, 15 (7). e1900571. doi:10.1002/biot.201900571
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WRAP-Production-substituted-styrene-bioproducts-lignin-lignocellulose-2020.pdf - Accepted Version - Requires a PDF viewer. Download (436Kb) | Preview |
Official URL: https://doi.org/10.1002/biot.201900571
Abstract
Ferulic acid is a renewable chemical found in lignocellulose from grasses such as wheat straw and sugarcane. Pseudomonas putida is able to liberate and metabolize ferulic acid from plant biomass. Deletion of the hydroxycinnamoyl-CoA hydratase-lyase gene (ech) produced a strain of P. putida unable to utilize ferulic and p-coumaric acid, which is able to accumulate ferulic acid and p-coumaric acid from wheat straw or sugar cane bagasse. Further engineering of this strain saw the replacement of ech with the phenolic acid decarboxylase padC, which converts p-coumaric and ferulic acid into 4-vinylphenol and the flavor agent 4-vinylguaiacol, respectively. The engineered strain containing padC is able to generate 4-vinylguaiacol and 4-vinylphenol from media containing lignocellulose or Green Value Protobind lignin as feedstock, and does not require the addition of an exogenous inducer molecule. Biopolymerization of 4-vinylguaiacol and 4-vinylcatechol styrene products is also carried out, using Trametes versicolor laccase, to generate "biopolystyrene" materials on small scale. [Abstract copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.]
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Chemistry | ||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Pseudomonas, Metabolism, Hydroxycitric acid, Biological products | ||||||||||||
| Journal or Publication Title: | Biotechnology Journal | ||||||||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||
| ISSN: | 1860-6768 | ||||||||||||
| Official Date: | July 2020 | ||||||||||||
| Dates: |
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| Volume: | 15 | ||||||||||||
| Number: | 7 | ||||||||||||
| Article Number: | e1900571 | ||||||||||||
| DOI: | 10.1002/biot.201900571 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Publisher Statement: | "This is the peer reviewed version of the following article: Williamson, J. J., Bahrin, N., Hardiman, E. M., Bugg, T. D. H., Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440. Biotechnol. J. 2020, 1900571. https://doi.org/10.1002/biot.201900571, which has been published in final form at https://doi.org/10.1002/biot.201900571 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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