Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Production of substituted styrene bioproducts from lignin and lignocellulose using engineered pseudomonas putida KT2440

Tools
- Tools
+ Tools

Williamson, James J., Bahrin, Nurfariza, Hardiman, Elizabeth M. and Bugg, Timothy D. H. (2020) Production of substituted styrene bioproducts from lignin and lignocellulose using engineered pseudomonas putida KT2440. Biotechnology Journal . e1900571. doi:10.1002/biot.201900571 (In Press)

[img] PDF
WRAP-Production-substituted-styrene-bioproducts-lignin-lignocellulose-2020.pdf - Accepted Version
Embargoed item. Restricted access to Repository staff only until 2 June 2021. Contact author directly, specifying your specific needs. - Requires a PDF viewer.

Download (436Kb)
Official URL: https://doi.org/10.1002/biot.201900571

Request Changes to record.

Abstract

Ferulic acid is a renewable chemical found in lignocellulose from grasses such as wheat straw and sugarcane. Pseudomonas putida is able to liberate and metabolize ferulic acid from plant biomass. Deletion of the hydroxycinnamoyl-CoA hydratase-lyase gene (ech) produced a strain of P. putida unable to utilize ferulic and p-coumaric acid, which is able to accumulate ferulic acid and p-coumaric acid from wheat straw or sugar cane bagasse. Further engineering of this strain saw the replacement of ech with the phenolic acid decarboxylase padC, which converts p-coumaric and ferulic acid into 4-vinylphenol and the flavor agent 4-vinylguaiacol, respectively. The engineered strain containing padC is able to generate 4-vinylguaiacol and 4-vinylphenol from media containing lignocellulose or Green Value Protobind lignin as feedstock, and does not require the addition of an exogenous inducer molecule. Biopolymerization of 4-vinylguaiacol and 4-vinylcatechol styrene products is also carried out, using Trametes versicolor laccase, to generate "biopolystyrene" materials on small scale. [Abstract copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.]

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Q Science > QR Microbiology
Divisions: Faculty of Science > Chemistry
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Pseudomonas, Metabolism, Hydroxycitric acid, Biological products
Journal or Publication Title: Biotechnology Journal
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 1860-6768
Official Date: 2020
Dates:
DateEvent
2020Published
2 June 2020Available
30 May 2020Accepted
Date of first compliant deposit: 18 June 2020
Article Number: e1900571
DOI: 10.1002/biot.201900571
Status: Peer Reviewed
Publication Status: In Press
Publisher Statement: "This is the peer reviewed version of the following article: Williamson, J. J., Bahrin, N., Hardiman, E. M., Bugg, T. D. H., Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440. Biotechnol. J. 2020, 1900571. https://doi.org/10.1002/biot.201900571, which has been published in final form at https://doi.org/10.1002/biot.201900571 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/P01738X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/H004270/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIEDMajlis Amanah Rakyathttp://dx.doi.org/10.13039/501100009233

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us