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Pulling of tethers from the cell plasma membrane using optical tweezers
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Köster, Darius Vasco (2020) Pulling of tethers from the cell plasma membrane using optical tweezers. In: Blouin, C., (ed.) Caveolae. Methods in Molecular Biology, 2169 . Humana, New York, NY: Springer, pp. 167-174. ISBN 9781071607312
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WRAP-Pulling-tethers-cell-plasma-membrane-optical-tweezers-Koster-2020.pdf - Accepted Version - Requires a PDF viewer. Download (805Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/978-1-0716-0732-9_15
Abstract
Here, we describe how to extract tethers or lipid membrane nanotubes from the plasma membrane of cells using optical tweezers. This technique allows measuring the force required to hold the membrane tether at a constant length, which is related to the cell membrane tension. Following the evolution of this force during mechanical or chemical perturbations of the cell gives insight about the regulation of cell membrane tension. By pulling very long membrane tethers, one can also probe the membrane reservoir of a cell and a sudden rise in the tether force is usually due to the depletion of excess membranes stored in membrane folds or invaginations.
| Item Type: | Book Item | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history Q Science > QP Physiology T Technology > TK Electrical engineering. Electronics Nuclear engineering |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
| Library of Congress Subject Headings (LCSH): | Optical tweezers, , Membrane lipids, Cell membranes | ||||
| Series Name: | Methods in Molecular Biology | ||||
| Journal or Publication Title: | Caveolae | ||||
| Publisher: | Springer | ||||
| Place of Publication: | Humana, New York, NY | ||||
| ISBN: | 9781071607312 | ||||
| ISSN: | 1064-3745 | ||||
| Book Title: | Caveolae | ||||
| Editor: | Blouin, C. | ||||
| Official Date: | 17 June 2020 | ||||
| Dates: |
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| Volume: | 2169 | ||||
| Page Range: | pp. 167-174 | ||||
| DOI: | 10.1007/978-1-0716-0732-9_15 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Date of first compliant deposit: | 29 June 2020 | ||||
| Date of first compliant Open Access: | 17 June 2022 |
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