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Isolation and chacterisation of Klebsiella phages for phage therapy

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Townsend, Eleanor, Kelly, Lucy, Gannon, Lucy, Muscatt, George, Dunstan, Rhys, Michniewski, Slawomir, Sapkota, Hari, Kiljunen, Saija J., Kolsi, Anna, Skurnik, Mikael, Lithgow, Trevor, Millard, Andrew D. and Jameson, Eleanor (2021) Isolation and chacterisation of Klebsiella phages for phage therapy. PHAGE Therapy, Applications, and Research, 2 (1). pp. 26-42. doi:10.1089/phage.2020.0046

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Official URL: https://doi.org/10.1089/phage.2020.0046

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Abstract

Klebsiella is a clinically important pathogen causing a variety of antimicrobial resistant infections, in both community and nosocomial settings, particularly pneumonia, urinary tract infection and septicaemia. We report the successful isolation and characterisation of 30 diverse Klebsiella-infecting phages. The isolated phages are diverse, spanning six different phage families and nine genera. These phages comprise of both lysogenic and lytic lifestyles. Individual Klebsiella phage isolates infected 11 of 18 different Klebsiella capsule types across all six Klebsiella species tested. Our Klebsiella-infecting lytic phages are suitable for phage therapy, based on the criteria that they encode no known toxin or antimicrobial resistance genes. However, none of the characterised phages were able to suppress the growth of Klebsiella for more than 7 hours. This indicates that for successful phage therapy, a mixed cocktail of multiple phages is necessary to treat Klebsiella infections.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Klebsiella pneumoniae, Bacteriophages -- Therapeutic use, Drug resistance in microorganisms
Journal or Publication Title: PHAGE Therapy, Applications, and Research
Publisher: Mary Ann Liebert
ISSN: 2641-6530
Book Title: Isolation and chacterisation of Klebsiella phages for phage therapy
Official Date: 17 March 2021
Dates:
DateEvent
17 March 2021Published
Volume: 2
Number: 1
Page Range: pp. 26-42
DOI: 10.1089/phage.2020.0046
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Copyright Holders: © Eleanor M. Townsend et al. 2021; Published by Mary Ann Liebert, Inc.
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
Warwick Integrative Synthetic Biology early career fellowshipBiotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
Warwick Integrative Synthetic Biology early career fellowshipEngineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
Accelerator FundMonash Universityhttp://dx.doi.org/10.13039/501100001779
Accelerator FundUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
MR/T030062/1Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
PhD fellowshipBiotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
PhD fellowshipEngineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
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