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Detection of measles specific IgG in oral fluid using an FITC/anti-FITC IgG capture enzyme linked immunosorbent assay (GACELISA)
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UNSPECIFIED (1999) Detection of measles specific IgG in oral fluid using an FITC/anti-FITC IgG capture enzyme linked immunosorbent assay (GACELISA). JOURNAL OF VIROLOGICAL METHODS, 83 (1-2). pp. 135-144.
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Abstract
An IgG antibody capture enzyme linked immunosorbent assay (GACELISA) for the detection of measles specific IgG in oral fluid was developed using an FITC/anti-FITC amplification system. The GACELISA was evaluated by testing paired oral fluid and serum samples from 787 subjects in an epidemiological study of measles in rural Ethiopia. Oral fluids were tested by GACELISA and corresponding serum samples by a sensitive indirect ELISA for measles IgG (Behring Enzygnost). By comparison with the serum measles IgG assay, the oral fluid GACELISA had a sensitivity of 97.4% (95% confidence intervals: 95.9, 98.2) and a specificity of 90.0% (81.9, 94.3), with no significant differences observed by age group. Total IgG concentrations were measured on a subset of 160 oral fluids by an in-house ELISA. This showed that false negative GACELISA results tended to occur in samples with low concentrations of total IgG, although the trend was not statistically significant. It is concluded that the overall performance of the GACELISA was satisfactory, showing close agreement to the serum ELISA, and has potential to serve as an easily transferable tool for large scale epidemiological studies as required for the World Health Organisation's programme for the global control of measles. (C) 1999 Elsevier Science B.V. All rights reserved.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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| Journal or Publication Title: | JOURNAL OF VIROLOGICAL METHODS | ||||
| Publisher: | ELSEVIER SCIENCE BV | ||||
| ISSN: | 0166-0934 | ||||
| Official Date: | December 1999 | ||||
| Dates: |
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| Volume: | 83 | ||||
| Number: | 1-2 | ||||
| Number of Pages: | 10 | ||||
| Page Range: | pp. 135-144 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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