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Rapid functionalisation and detection of viruses via a novel Ca2+-mediated virus-DNA interaction

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Robb, Nicole C., Taylor, Jonathan M., Kent, Amy, Pambos, Oliver J., Gilboa, Barak, Evangelidou, Maria, Mentis, Alexios-Fotios A. and Kapanidis, Achillefs N. (2019) Rapid functionalisation and detection of viruses via a novel Ca2+-mediated virus-DNA interaction. Scientific Reports, 9 (1). 16219. doi:10.1038/s41598-019-52759-5

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Official URL: http://dx.doi.org/10.1038/s41598-019-52759-5

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Abstract

Current virus detection methods often take significant time or can be limited in sensitivity and specificity. The increasing frequency and magnitude of viral outbreaks in recent decades has resulted in an urgent need for diagnostic methods that are facile, sensitive, rapid and inexpensive. Here, we describe and characterise a novel, calcium-mediated interaction of the surface of enveloped viruses with DNA, that can be used for the functionalisation of intact virus particles via chemical groups attached to the DNA. Using DNA modified with fluorophores, we have demonstrated the rapid and sensitive labelling and detection of influenza and other viruses using single-particle tracking and particle-size determination. With this method, we have detected clinical isolates of influenza in just one minute, significantly faster than existing rapid diagnostic tests. This powerful technique is easily extendable to a wide range of other enveloped pathogenic viruses and holds significant promise as a future diagnostic tool.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QP Physiology
Q Science > QR Microbiology
R Medicine > RA Public aspects of medicine
R Medicine > RC Internal medicine
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Viruses -- Isolation, Virology -- Methodology, Virus diseases -- Diagnosis, DNA-ligand interactions , Transmission electron microscopy
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Official Date: 7 November 2019
Dates:
DateEvent
7 November 2019Published
22 October 2019Accepted
Date of first compliant deposit: 28 July 2020
Volume: 9
Number: 1
Article Number: 16219
DOI: 10.1038/s41598-019-52759-5
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
110164/Z/15/Z Wellcome Trusthttp://dx.doi.org/10.13039/100010269
MR/N010744/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
Dorothy Hodgkin Research Fellowship DKR00620Royal Societyhttp://dx.doi.org/10.13039/501100000288
Educational scholarshipAlexander S. Onassis Public Benefit Foundationhttp://dx.doi.org/10.13039/501100005302

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