The Library
Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics
Tools
Robb, Nicole C., te Velthuis, Aartjan J. W., Fodor, Ervin and Kapanidis, Achillefs N. (2019) Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics. Nucleic Acids Research, 47 (12). pp. 6466-6477. doi:10.1093/nar/gkz313 ISSN 0305-1048.
|
PDF
WRAP-Real-time-analysis-single-influenza-virus-replication-Robb-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (7Mb) | Preview |
Official URL: http://dx.doi.org/10.1093/nar/gkz313
Abstract
The viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; further, little is known about cRNA promoter conformations. To probe the RNA conformations adopted during initial replication, we monitored single, surface-immobilized vRNA and cRNA initiation complexes in real-time. Our results show that, while the 3′ terminus of the vRNA promoter exists in dynamic equilibrium between pre-initiation and initiation conformations, the cRNA promoter exhibited very limited dynamics. Two residues in the proximal 3′ region of the cRNA promoter (residues absent in the vRNA promoter) allowed the cRNA template strand to reach further into the active site, limiting promoter dynamics. Our results highlight promoter-dependent differences in influenza initiation mechanisms, and advance our understanding of virus replication.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
||||||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Influenza viruses, Influenza viruses -- Reproduction, RNA | ||||||||||||||||||||||||||||||
Journal or Publication Title: | Nucleic Acids Research | ||||||||||||||||||||||||||||||
Publisher: | Oxford University Press | ||||||||||||||||||||||||||||||
ISSN: | 0305-1048 | ||||||||||||||||||||||||||||||
Official Date: | July 2019 | ||||||||||||||||||||||||||||||
Dates: |
|
||||||||||||||||||||||||||||||
Volume: | 47 | ||||||||||||||||||||||||||||||
Number: | 12 | ||||||||||||||||||||||||||||||
Page Range: | pp. 6466-6477 | ||||||||||||||||||||||||||||||
DOI: | 10.1093/nar/gkz313 | ||||||||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||
Date of first compliant deposit: | 28 July 2020 | ||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 28 July 2020 | ||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year