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Slow‐decaying presynaptic calcium dynamics gate long‐lasting asynchronous release at the hippocampal mossy fiber to CA3 pyramidal cell synapse
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Chamberland, Simon, Timofeeva, Yulia, Evstratova, Alesya, Norman, Christopher, Volynski, Kirill and Tóth, Katalin (2020) Slow‐decaying presynaptic calcium dynamics gate long‐lasting asynchronous release at the hippocampal mossy fiber to CA3 pyramidal cell synapse. Synapse, 74 (12). e22178. doi:10.1002/syn.22178 ISSN 1098-2396.
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Official URL: https://doi.org/10.1002/syn.22178
Abstract
Action potentials trigger two modes of neurotransmitter release, with a fast synchronous component and a temporally delayed asynchronous release. Asynchronous release contributes to information transfer at synapses, including at the hippocampal mossy fiber (MF) to CA3 pyramidal cell synapse where it controls the timing of postsynaptic CA3 pyramidal neuron firing. Here, we identified and characterized the main determinants of asynchronous release at the MF–CA3 synapse. We found that asynchronous release at MF–CA3 synapses can last on the order of seconds following repetitive MF stimulation. Elevating the stimulation frequency or the external Ca2+ concentration increased the rate of asynchronous release, thus, arguing that presynaptic Ca2+ dynamics is the major determinant of asynchronous release rate. Direct MF bouton Ca2+ imaging revealed slow Ca2+ decay kinetics of action potential (AP) burst‐evoked Ca2+ transients. Finally, we observed that asynchronous release was preferentially mediated by Ca2+ influx through P/Q‐type voltage‐gated Ca2+ channels, while the contribution of N‐type VGCCs was limited. Overall, our results uncover the determinants of long‐lasting asynchronous release from MF terminals and suggest that asynchronous release could influence CA3 pyramidal cell firing up to seconds following termination of granule cell bursting.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QM Human anatomy Q Science > QP Physiology T Technology > TK Electrical engineering. Electronics Nuclear engineering T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Research Centres > Centre for Complexity Science Faculty of Science, Engineering and Medicine > Science > Computer Science |
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SWORD Depositor: | Library Publications Router | |||||||||
Library of Congress Subject Headings (LCSH): | Asynchronous transfer mode, Calcium, Hippocampus (Brain), Presynaptic receptors | |||||||||
Journal or Publication Title: | Synapse | |||||||||
Publisher: | Wiley | |||||||||
ISSN: | 1098-2396 | |||||||||
Official Date: | December 2020 | |||||||||
Dates: |
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Volume: | 74 | |||||||||
Number: | 12 | |||||||||
Article Number: | e22178 | |||||||||
DOI: | 10.1002/syn.22178 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 31 July 2020 | |||||||||
Date of first compliant Open Access: | 4 August 2020 | |||||||||
RIOXX Funder/Project Grant: |
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