UNSPECIFIED (1999) Carboxylase genes of Sulfolobus metallicus. ARCHIVES OF MICROBIOLOGY, 172 (6). pp. 349-353. ISSN 0302-8933

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Abstract

Carbon dioxide limitation of Sulfolobus metallicus resulted in increased cellular concentrations of polypeptides that were predicted to be biotin carboxylase and biotin carboxyl-carrier-protein components of a protein complex. These polypeptides were coeluted from a native polyacrylamide gel and were estimated at 19 and 59 kDa after separation by denaturing gel electrophoresis. Their encoding genes were identified, sequenced and shown to code for polypeptides of 18,580 and 58,235 Da with similarities to biotin carboxyl carrier proteins and biotin carboxylases, respectively. The genes overlapped at the second of two stop codons that terminated the carboxylase gene. A third gene occurred on the opposite strand, 293 bp upstream of the biotin carboxylase gene. Its deduced amino acid sequence was similar to those of carboxyl transferase subunits of carboxylase enzymes, in particular to those of the propionyl-CoA carboxylases. It is proposed that the three described genes could encode the key enzyme complex responsible for carbon dioxide fixation during autotrophic growth of the thermoacidophilic archaea.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	ARCHIVES OF MICROBIOLOGY
Publisher:	SPRINGER VERLAG
ISSN:	0302-8933
Date:	December 1999
Volume:	172
Number:	6
Number of Pages:	5
Page Range:	pp. 349-353
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/13975

Data sourced from Thomson Reuters' Web of Knowledge

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