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Inhibition of cell membrane ingression at the division site by cell wall in fission yeast
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Chew, Ting Gang, Lim, Tzer Chyn, Osaki, Yumi, Huang, Junqi, Kamnev, Anton, Osumi, Masako and Balasubramanian, Mohan K. (2020) Inhibition of cell membrane ingression at the division site by cell wall in fission yeast. Molecular Biology of the Cell, 31 (21). pp. 2306-2314. doi:10.1091/mbc.E20-04-0245 ISSN 1059-1524.
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WRAP-Inhibition-cell-membrane-ingression-division-site-cell-wall-Balasubramanian-2020.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1276Kb) | Preview |
Official URL: https://doi.org/10.1091/mbc.E20-04-0245
Abstract
Eukaryotic cells assemble an actomyosin ring during cytokinesis to function as a force-generating machine to drive membrane invagination, and to counteract the intracellular pressure and the cell surface tension. It is unclear whether additional factors such as the extracellular matrix (cell wall in yeasts and fungi) affect the actomyosin ring contraction. While studying the fission yeast β-glucan synthase mutant cps1-191, which is defective in division septum synthesis and actomyosin ring contraction, we found that significantly weakening of the extracellular glycan matrix caused the spheroplasts to divide at the non-permissive condition. This division was dependent on a functional actomyosin ring and vesicular trafficking, but independent of normal septum synthesis. cps1-191 cells with weakened extracellular glycan matrix divide non-medially with a much slower ring contraction rate compared to wild type cells under similar conditions, which we term as cytofission. Interestingly, the high turgor pressure appears to play minimal roles in inhibiting ring contraction in cps1-191 mutants as decreasing the turgor pressure alone does not enable cytofission. We propose that during cytokinesis, the extracellular glycan matrix restricts actomyosin ring contraction and membrane ingression, and remodeling of the extracellular components through division septum synthesis relieves the inhibition and facilitates actomyosin ring contraction.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics | ||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Cytokinesis, Eukaryotic cells, Fungal cell walls, Cell cycle, Schizosaccharomyces pombe | ||||||||||||
Journal or Publication Title: | Molecular Biology of the Cell | ||||||||||||
Publisher: | American Society for Cell Biology | ||||||||||||
ISSN: | 1059-1524 | ||||||||||||
Book Title: | Inhibition of cell membrane ingression at the division site by cell wall in fission yeast | ||||||||||||
Official Date: | 1 October 2020 | ||||||||||||
Dates: |
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Volume: | 31 | ||||||||||||
Number: | 21 | ||||||||||||
Page Range: | pp. 2306-2314 | ||||||||||||
DOI: | 10.1091/mbc.E20-04-0245 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Reuse Statement (publisher, data, author rights): | (https://creativecommons.org/licenses/by/4.0), Others may reuse the work for any purpose, including commercially, but it cannot be shared with others in adapted form, and credit must be provided to the authors. | ||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
Date of first compliant deposit: | 5 August 2020 | ||||||||||||
Date of first compliant Open Access: | 11 August 2021 | ||||||||||||
RIOXX Funder/Project Grant: |
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