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Pre-analytical and analytical variables that influence urinary volatile organic compound measurements
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McFarlane, Michael J., Mozdiak, Ella , Daulton, Emma, Arasaradnam, Ramesh P., Covington, James A. and Nwokolo, Chuka U. (2020) Pre-analytical and analytical variables that influence urinary volatile organic compound measurements. PLoS One, 15 (7). e0236591. doi:10.1371/journal.pone.0236591
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WRAP-Pre-analytical-influence-urinary-volatile-organic-measurements-Covington-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3592Kb) | Preview |
Official URL: https://doi.org/10.1371/journal.pone.0236591
Abstract
There has been rapidly accelerating interest in the utilization of volatile organic compounds (VOCs) as non-invasive methods for rapid point-of-care medical diagnostics. There is widespread variation in analytical methods and protocols, with little understanding of the effects of sample storage on VOC profiles. This study aimed to determine the effects on VOC profiles of different storage times, at room temperature, prior to freezing, of sealed urine samples from healthy individuals. Analysis using Field Asymmetric Ion Motility Spectrometry (FAIMS) determined the alterations in VOC and total ion count profiles as a result of increasing room temperature storage times. Results indicated that increasing exposure time to room temperature prior to freezing had a threefold effect. Firstly, increased urinary VOC profile variability, with a plateau phase between 12 and 48 hours, before further degradation. Secondly, an increase in total ion count with time exposed to room temperature. Finally, a deterioration in VOCs with each sample run during the analysis process. This provides new insight into the effect of storage of urine samples for VOC analysis using FAIMS technology. Results of this study provide a recommendation for a 12-hour maximum duration at room temperature prior to storage.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | T Technology > TD Environmental technology. Sanitary engineering | ||||||
| Divisions: | Faculty of Science > Engineering | ||||||
| SWORD Depositor: | Library Publications Router | ||||||
| Library of Congress Subject Headings (LCSH): | Volatile organic compounds, Biochemical markers, Urine | ||||||
| Journal or Publication Title: | PLoS One | ||||||
| Publisher: | Public Library of Science | ||||||
| ISSN: | 1932-6203 | ||||||
| Official Date: | 31 July 2020 | ||||||
| Dates: |
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| Volume: | 15 | ||||||
| Number: | 7 | ||||||
| Article Number: | e0236591 | ||||||
| DOI: | 10.1371/journal.pone.0236591 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Publisher Statement: | ** From PubMed via Jisc Publications Router ** History: received 24-03-2020; accepted 08-07-2020. | ||||||
| Access rights to Published version: | Open Access |
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