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First cryo-scanning electron microscopy images and X-ray microanalyses of mucoromycotinian fine root endophytes in vascular plants
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Albornoz, Felipe E., Hayes, Patrick E., Orchard, Suzanne, Clode, Peta L., Nazeri, Nazanin, Standish, Rachel J., Dickie, Ian A., Bending, G. D., Hilton, Sally and Ryan, Megan H. (2020) First cryo-scanning electron microscopy images and X-ray microanalyses of mucoromycotinian fine root endophytes in vascular plants. Frontiers in Microbiology, 11 . 2018. doi:10.3389/fmicb.2020.02018 ISSN 1664-302X.
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Official URL: http://doi.org/10.3389/fmicb.2020.02018
Abstract
Aims. Arbuscule-producing fine root endophytes (FRE) (previously incorrectly Glomus tenue) were recently placed within subphylum Mucoromycotina; the first report of arbuscules outside subphylum Glomeromycotina. Here, we aimed to estimate nutrient concentrations in plant and fungal structures of FRE and to test the utility of cryo-scanning electron microscopy (cryoSEM) for studying these fungi.
Methods. To do so, we used replicated cryoSEM and X-ray microanalysis of heavily colonized roots of Trifolium subterraneum.
Results. Intercellular hyphae and hyphae in developed arbuscules were consistently very thin; 1.35 ± 0.03 µm and 0.99 ± 0.03 µm in diameter, respectively (mean ± SE). Several intercellular hyphae were often adjacent to each other forming ‘hyphal ropes’. Developed arbuscules showed higher phosphorus concentrations than senesced arbuscules and non-colonized structures. Senesced arbuscules showed greatly elevated concentrations of calcium and magnesium.
Conclusions. While uniformly thin hyphae and hyphal ropes are distinct features of FRE, the morphology of fully developed arbuscules, elevated phosphorus in fungal structures, and accumulation of calcium with loss of structural integrity in senesced arbuscules are similar to glomeromycotinian fungi. Thus, we provide evidence that FRE may respond to similar host-plant signals or that the host plant may employ a similar mechanism of association with FRE and AMF.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QK Botany S Agriculture > SB Plant culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
Library of Congress Subject Headings (LCSH): | Plant-fungus relationships, Subterranean clover , Endophytes, Scanning electron microscopy | ||||||||||||
Journal or Publication Title: | Frontiers in Microbiology | ||||||||||||
Publisher: | Frontiers Research Foundation | ||||||||||||
ISSN: | 1664-302X | ||||||||||||
Official Date: | 3 September 2020 | ||||||||||||
Dates: |
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Volume: | 11 | ||||||||||||
Article Number: | 2018 | ||||||||||||
DOI: | 10.3389/fmicb.2020.02018 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 14 August 2020 | ||||||||||||
Date of first compliant Open Access: | 24 September 2020 | ||||||||||||
RIOXX Funder/Project Grant: |
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