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Data for Polymer-stabilized sialylated nanoparticles : synthesis, optimization, and differential binding to influenza hemagglutinins

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Richards, Sarah-Jane, Baker, Alexander, Walker, Marc and Gibson, Matthew I. (2020) Data for Polymer-stabilized sialylated nanoparticles : synthesis, optimization, and differential binding to influenza hemagglutinins. [Dataset]

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Abstract

During influenza infection, hemagglutinins (HAs) on the viral surface bind to sialic acids on the host cell's surface. While all HAs bind sialic acids, human influenza targets terminal α2,6 sialic acids and avian influenza targets α2,3 sialic acids. For interspecies transmission (zoonosis), HA must mutate to adapt to these differences. Here, multivalent gold nanoparticles bearing either α2,6- or α2,3-sialyllactosamine have been developed to interrogate a panel of HAs from pathogenic human, low pathogenic avian, and other species' influenza. This method exploits the benefits of multivalent glycan presentation compared to monovalent presentation to increase affinity and investigate how multivalency affects selectivity. Using a library-orientated approach, parameters including polymer coating and core diameter were optimized for maximal binding and specificity were probed using galactosylated particles and a panel of biophysical techniques [ultraviolet-visible spectroscopy, dynamic light scattering, and biolayer interferometry]. The optimized particles were then functionalized with sialyllactosamine and their binding analyzed against a panel of HAs derived from pathogenic influenza strains including low pathogenic avian strains. This showed significant specificity crossover, which is not observed in monovalent formats, with binding of avian HAs to human sialic acids and in agreement with alternate assay formats. These results demonstrate that precise multivalent presentation is essential to dissect the interactions of HAs and may aid the discovery of tools for disease and zoonosis transmission.

Item Type: Dataset
Subjects: Q Science > QP Physiology
Q Science > QR Microbiology
R Medicine > RA Public aspects of medicine
R Medicine > RC Internal medicine
R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Science > Chemistry
Type of Data: Experimental data
Library of Congress Subject Headings (LCSH): Influenza, Hemagglutinin, Multivalent molecules, Binding sites (Biochemistry)
Publisher: Department of Chemistry
Official Date: 18 August 2020
Dates:
DateEvent
18 August 2020Created
Date of first compliant deposit: 18 August 2020
Status: Not Peer Reviewed
Publication Status: Published
Media of Output: .vms
Access rights to Published version: Open Access
Copyright Holders: University of Warwick
Description:

Data record consists of a zip archive containing raw XPS data in .vms format.

RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
CRYOMAT 638661[ERC] Horizon 2020 Framework Programmehttp://dx.doi.org/10.13039/100010661
UNSPECIFIEDRoyal Societyhttp://dx.doi.org/10.13039/501100000288
BB/M02878X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/M01116X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIEDIceni Diagnosticshttps://www.icenidiagnostics.com/
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Contributors:
ContributionNameContributor ID
DepositorGibson, Matthew I.32015

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