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The hydroxyquinol degradation pathway in Rhodococcus jostii RHA1 and Agrobacterium species is an alternative pathway for degradation of protocatechuic acid and lignin fragments
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Spence, Edward M., Scott, Heather T., Dumond, Louison, Calvo-Bado, Leo A., Monaco, Sabrina di, Williamson, James J., Persinoti, Gabriela F., Squina, Fabio M. and Bugg, Timothy D. H. (2020) The hydroxyquinol degradation pathway in Rhodococcus jostii RHA1 and Agrobacterium species is an alternative pathway for degradation of protocatechuic acid and lignin fragments. Applied and Environmental Microbiology, 86 (19). e01561--20. doi:10.1128/AEM.01561-20 ISSN 0099-2240.
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WRAP-characterisation-hydroxyquinol-degradation-pathway-Rhodococcus-jostii-RHA1-Agrobacterium-sp.-Bugg-2020.pdf - Accepted Version - Requires a PDF viewer. Download (2184Kb) | Preview |
Official URL: http://dx.doi.org/10.1128/AEM.01561-20
Abstract
Deletion of the pcaHG genes encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1 gives a gene deletion strain still able to grow on protocatechuic acid as sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on media containing vanillin or protocatechuic acid indicated the formation of hydroxyquinol (benzene-1,2,4-triol) as a downstream product. Gene cluster ro01857-ro01860 in Rhodococcus jostii RHA1 contains genes encoding hydroxyquinol 1,2-dioxygenase and maleylacetate reductase for degradation of hydroxyquinol but also putative mono-oxygenase (ro01860) and putative decarboxylase (ro01859) genes, and a similar gene cluster is found in the genome of lignin-degrading Agrobacterium sp.. Recombinant R. jostii mono-oxygenase and decarboxylase enzymes were in combination found to convert protocatechuic acid to hydroxyquinol. Hence an alternative pathway for degradation of protocatechuic acid via oxidative decarboxylation to hydroxyquinol is proposed.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QK Botany Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Microbial metabolism, Phenolic acids , Lignin , Lignin -- Biodegradation | |||||||||||||||
Journal or Publication Title: | Applied and Environmental Microbiology | |||||||||||||||
Publisher: | American Society for Microbiology | |||||||||||||||
ISSN: | 0099-2240 | |||||||||||||||
Official Date: | 17 September 2020 | |||||||||||||||
Dates: |
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Volume: | 86 | |||||||||||||||
Number: | 19 | |||||||||||||||
Page Range: | e01561--20 | |||||||||||||||
DOI: | 10.1128/AEM.01561-20 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||
Date of first compliant deposit: | 26 August 2020 | |||||||||||||||
Date of first compliant Open Access: | 31 January 2021 | |||||||||||||||
RIOXX Funder/Project Grant: |
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